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Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China
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| Zeitschriftentitel: | PeerJ |
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| Personen und Körperschaften: | , , , , , |
| In: | PeerJ, 7, 2019, S. e7307 |
| Format: | E-Article |
| Sprache: | Englisch |
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PeerJ
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| author_facet |
Wang, Siyu Guo, Hongbo Li, JiaJia Li, Wei Wang, Qin Yu, Xiaodan Wang, Siyu Guo, Hongbo Li, JiaJia Li, Wei Wang, Qin Yu, Xiaodan |
|---|---|
| author |
Wang, Siyu Guo, Hongbo Li, JiaJia Li, Wei Wang, Qin Yu, Xiaodan |
| spellingShingle |
Wang, Siyu Guo, Hongbo Li, JiaJia Li, Wei Wang, Qin Yu, Xiaodan PeerJ Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology General Medicine General Neuroscience |
| author_sort |
wang, siyu |
| spelling |
Wang, Siyu Guo, Hongbo Li, JiaJia Li, Wei Wang, Qin Yu, Xiaodan 2167-8359 PeerJ General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology General Medicine General Neuroscience http://dx.doi.org/10.7717/peerj.7307 <jats:sec> <jats:title>Background</jats:title> <jats:p>Distinguishing among species in the genus <jats:italic>Lepista</jats:italic> is difficult because of their similar morphologies.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>To identify a suitable DNA barcode for identification of <jats:italic>Lepista</jats:italic> species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight <jats:italic>Lepista</jats:italic> species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus <jats:italic>Lepista</jats:italic>. In addition, a phylogenetic tree based on the ITS region showed that the tested eight <jats:italic>Lepista</jats:italic> species, including two unrecognized species, formed eight separate and well-supported clades.</jats:p> </jats:sec> Evaluation of five regions as DNA barcodes for identification of <i>Lepista</i> species (Tricholomataceae, Basidiomycota) from China PeerJ |
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| title |
Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_unstemmed |
Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_full |
Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_fullStr |
Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_full_unstemmed |
Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_short |
Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_sort |
evaluation of five regions as dna barcodes for identification of <i>lepista</i> species (tricholomataceae, basidiomycota) from china |
| topic |
General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology General Medicine General Neuroscience |
| url |
http://dx.doi.org/10.7717/peerj.7307 |
| publishDate |
2019 |
| physical |
e7307 |
| description |
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Distinguishing among species in the genus <jats:italic>Lepista</jats:italic> is difficult because of their similar morphologies.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods</jats:title>
<jats:p>To identify a suitable DNA barcode for identification of <jats:italic>Lepista</jats:italic> species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight <jats:italic>Lepista</jats:italic> species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus <jats:italic>Lepista</jats:italic>. In addition, a phylogenetic tree based on the ITS region showed that the tested eight <jats:italic>Lepista</jats:italic> species, including two unrecognized species, formed eight separate and well-supported clades.</jats:p>
</jats:sec> |
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| author | Wang, Siyu, Guo, Hongbo, Li, JiaJia, Li, Wei, Wang, Qin, Yu, Xiaodan |
| author_facet | Wang, Siyu, Guo, Hongbo, Li, JiaJia, Li, Wei, Wang, Qin, Yu, Xiaodan, Wang, Siyu, Guo, Hongbo, Li, JiaJia, Li, Wei, Wang, Qin, Yu, Xiaodan |
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| description | <jats:sec> <jats:title>Background</jats:title> <jats:p>Distinguishing among species in the genus <jats:italic>Lepista</jats:italic> is difficult because of their similar morphologies.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>To identify a suitable DNA barcode for identification of <jats:italic>Lepista</jats:italic> species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight <jats:italic>Lepista</jats:italic> species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus <jats:italic>Lepista</jats:italic>. In addition, a phylogenetic tree based on the ITS region showed that the tested eight <jats:italic>Lepista</jats:italic> species, including two unrecognized species, formed eight separate and well-supported clades.</jats:p> </jats:sec> |
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| spelling | Wang, Siyu Guo, Hongbo Li, JiaJia Li, Wei Wang, Qin Yu, Xiaodan 2167-8359 PeerJ General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology General Medicine General Neuroscience http://dx.doi.org/10.7717/peerj.7307 <jats:sec> <jats:title>Background</jats:title> <jats:p>Distinguishing among species in the genus <jats:italic>Lepista</jats:italic> is difficult because of their similar morphologies.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>To identify a suitable DNA barcode for identification of <jats:italic>Lepista</jats:italic> species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight <jats:italic>Lepista</jats:italic> species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus <jats:italic>Lepista</jats:italic>. In addition, a phylogenetic tree based on the ITS region showed that the tested eight <jats:italic>Lepista</jats:italic> species, including two unrecognized species, formed eight separate and well-supported clades.</jats:p> </jats:sec> Evaluation of five regions as DNA barcodes for identification of <i>Lepista</i> species (Tricholomataceae, Basidiomycota) from China PeerJ |
| spellingShingle | Wang, Siyu, Guo, Hongbo, Li, JiaJia, Li, Wei, Wang, Qin, Yu, Xiaodan, PeerJ, Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, General Medicine, General Neuroscience |
| title | Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_full | Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_fullStr | Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_full_unstemmed | Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_short | Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| title_sort | evaluation of five regions as dna barcodes for identification of <i>lepista</i> species (tricholomataceae, basidiomycota) from china |
| title_unstemmed | Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China |
| topic | General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, General Medicine, General Neuroscience |
| url | http://dx.doi.org/10.7717/peerj.7307 |