Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China

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Zeitschriftentitel:	PeerJ
Personen und Körperschaften:	Wang, Siyu, Guo, Hongbo, Li, JiaJia, Li, Wei, Wang, Qin, Yu, Xiaodan
In:	PeerJ, 7, 2019, S. e7307
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	PeerJ
Schlagwörter:	General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology General Medicine General Neuroscience

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Zusammenfassung:	<jats:sec> <jats:title>Background</jats:title> <jats:p>Distinguishing among species in the genus <jats:italic>Lepista</jats:italic> is difficult because of their similar morphologies.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>To identify a suitable DNA barcode for identification of <jats:italic>Lepista</jats:italic> species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight <jats:italic>Lepista</jats:italic> species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus <jats:italic>Lepista</jats:italic>. In addition, a phylogenetic tree based on the ITS region showed that the tested eight <jats:italic>Lepista</jats:italic> species, including two unrecognized species, formed eight separate and well-supported clades.</jats:p> </jats:sec>
Umfang:	e7307
ISSN:	2167-8359
DOI:	10.7717/peerj.7307