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TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation
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| Zeitschriftentitel: | Journal of Cell Science |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Journal of Cell Science, 2012 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
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| Schlagwörter: |
| author_facet |
Heng, Yi-Wen Lim, Hong-Hwa Mina, Theresia Utomo, Prayudi Zhong, Shaoping Lim, Chwee-Teck Koh, Cheng-Gee Heng, Yi-Wen Lim, Hong-Hwa Mina, Theresia Utomo, Prayudi Zhong, Shaoping Lim, Chwee-Teck Koh, Cheng-Gee |
|---|---|
| author |
Heng, Yi-Wen Lim, Hong-Hwa Mina, Theresia Utomo, Prayudi Zhong, Shaoping Lim, Chwee-Teck Koh, Cheng-Gee |
| spellingShingle |
Heng, Yi-Wen Lim, Hong-Hwa Mina, Theresia Utomo, Prayudi Zhong, Shaoping Lim, Chwee-Teck Koh, Cheng-Gee Journal of Cell Science TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation Cell Biology |
| author_sort |
heng, yi-wen |
| spelling |
Heng, Yi-Wen Lim, Hong-Hwa Mina, Theresia Utomo, Prayudi Zhong, Shaoping Lim, Chwee-Teck Koh, Cheng-Gee 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.096818 <jats:p>The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. The actin cytoskeleton changes are most obvious in the adherent cells where the actin stress fibres are disassembled and the cortical actin network becomes more prominent with concomitant increase in cell rigidity as cells round up and enter mitosis. While the regulatory connections between the actin cytoskeleton and the early mitotic events are apparent, the mechanisms that govern these links are not well understood. Here, we report that LIMK1 and LIMK2, the downstream effectors of RhoA/ROCK, regulate centrosome integrity and astral microtubules organization respectively. Surprisingly, LIMK1 and cofilin are not involved downstream of RhoA/ROCK in the regulation of astral microtubule organization. Instead, we find LIMK2 acts via TPPP in the regulation of astral microtubule, while both LIMK1 and LIMK2 affect centrosome focusing. Both phenotypes are tightly coupled to spindle orientation in the mitotic cells. Thus, our results reveal a novel regulatory link between the actin cytoskeleton and the mitotic spindle during the early stages of mitosis.</jats:p> TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation Journal of Cell Science |
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Journal of Cell Science |
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| title |
TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_unstemmed |
TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_full |
TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_fullStr |
TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_full_unstemmed |
TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_short |
TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_sort |
tppp acts downstream of rhoa-rock-limk2 to regulate astral microtubule organization and spindle orientation |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1242/jcs.096818 |
| publishDate |
2012 |
| physical |
|
| description |
<jats:p>The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. The actin cytoskeleton changes are most obvious in the adherent cells where the actin stress fibres are disassembled and the cortical actin network becomes more prominent with concomitant increase in cell rigidity as cells round up and enter mitosis. While the regulatory connections between the actin cytoskeleton and the early mitotic events are apparent, the mechanisms that govern these links are not well understood. Here, we report that LIMK1 and LIMK2, the downstream effectors of RhoA/ROCK, regulate centrosome integrity and astral microtubules organization respectively. Surprisingly, LIMK1 and cofilin are not involved downstream of RhoA/ROCK in the regulation of astral microtubule organization. Instead, we find LIMK2 acts via TPPP in the regulation of astral microtubule, while both LIMK1 and LIMK2 affect centrosome focusing. Both phenotypes are tightly coupled to spindle orientation in the mitotic cells. Thus, our results reveal a novel regulatory link between the actin cytoskeleton and the mitotic spindle during the early stages of mitosis.</jats:p> |
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| author | Heng, Yi-Wen, Lim, Hong-Hwa, Mina, Theresia, Utomo, Prayudi, Zhong, Shaoping, Lim, Chwee-Teck, Koh, Cheng-Gee |
| author_facet | Heng, Yi-Wen, Lim, Hong-Hwa, Mina, Theresia, Utomo, Prayudi, Zhong, Shaoping, Lim, Chwee-Teck, Koh, Cheng-Gee, Heng, Yi-Wen, Lim, Hong-Hwa, Mina, Theresia, Utomo, Prayudi, Zhong, Shaoping, Lim, Chwee-Teck, Koh, Cheng-Gee |
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| description | <jats:p>The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. The actin cytoskeleton changes are most obvious in the adherent cells where the actin stress fibres are disassembled and the cortical actin network becomes more prominent with concomitant increase in cell rigidity as cells round up and enter mitosis. While the regulatory connections between the actin cytoskeleton and the early mitotic events are apparent, the mechanisms that govern these links are not well understood. Here, we report that LIMK1 and LIMK2, the downstream effectors of RhoA/ROCK, regulate centrosome integrity and astral microtubules organization respectively. Surprisingly, LIMK1 and cofilin are not involved downstream of RhoA/ROCK in the regulation of astral microtubule organization. Instead, we find LIMK2 acts via TPPP in the regulation of astral microtubule, while both LIMK1 and LIMK2 affect centrosome focusing. Both phenotypes are tightly coupled to spindle orientation in the mitotic cells. Thus, our results reveal a novel regulatory link between the actin cytoskeleton and the mitotic spindle during the early stages of mitosis.</jats:p> |
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| imprint | The Company of Biologists, 2012 |
| imprint_str_mv | The Company of Biologists, 2012 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | The Company of Biologists |
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| series | Journal of Cell Science |
| source_id | 49 |
| spelling | Heng, Yi-Wen Lim, Hong-Hwa Mina, Theresia Utomo, Prayudi Zhong, Shaoping Lim, Chwee-Teck Koh, Cheng-Gee 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.096818 <jats:p>The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. The actin cytoskeleton changes are most obvious in the adherent cells where the actin stress fibres are disassembled and the cortical actin network becomes more prominent with concomitant increase in cell rigidity as cells round up and enter mitosis. While the regulatory connections between the actin cytoskeleton and the early mitotic events are apparent, the mechanisms that govern these links are not well understood. Here, we report that LIMK1 and LIMK2, the downstream effectors of RhoA/ROCK, regulate centrosome integrity and astral microtubules organization respectively. Surprisingly, LIMK1 and cofilin are not involved downstream of RhoA/ROCK in the regulation of astral microtubule organization. Instead, we find LIMK2 acts via TPPP in the regulation of astral microtubule, while both LIMK1 and LIMK2 affect centrosome focusing. Both phenotypes are tightly coupled to spindle orientation in the mitotic cells. Thus, our results reveal a novel regulatory link between the actin cytoskeleton and the mitotic spindle during the early stages of mitosis.</jats:p> TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation Journal of Cell Science |
| spellingShingle | Heng, Yi-Wen, Lim, Hong-Hwa, Mina, Theresia, Utomo, Prayudi, Zhong, Shaoping, Lim, Chwee-Teck, Koh, Cheng-Gee, Journal of Cell Science, TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation, Cell Biology |
| title | TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_full | TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_fullStr | TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_full_unstemmed | TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_short | TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| title_sort | tppp acts downstream of rhoa-rock-limk2 to regulate astral microtubule organization and spindle orientation |
| title_unstemmed | TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1242/jcs.096818 |