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TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation
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| Zeitschriftentitel: | Journal of Cell Science |
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| Personen und Körperschaften: | , , , , , , |
| In: | Journal of Cell Science, 2012 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
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| Schlagwörter: |
| Zusammenfassung: | <jats:p>The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. The actin cytoskeleton changes are most obvious in the adherent cells where the actin stress fibres are disassembled and the cortical actin network becomes more prominent with concomitant increase in cell rigidity as cells round up and enter mitosis. While the regulatory connections between the actin cytoskeleton and the early mitotic events are apparent, the mechanisms that govern these links are not well understood. Here, we report that LIMK1 and LIMK2, the downstream effectors of RhoA/ROCK, regulate centrosome integrity and astral microtubules organization respectively. Surprisingly, LIMK1 and cofilin are not involved downstream of RhoA/ROCK in the regulation of astral microtubule organization. Instead, we find LIMK2 acts via TPPP in the regulation of astral microtubule, while both LIMK1 and LIMK2 affect centrosome focusing. Both phenotypes are tightly coupled to spindle orientation in the mitotic cells. Thus, our results reveal a novel regulatory link between the actin cytoskeleton and the mitotic spindle during the early stages of mitosis.</jats:p> |
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| ISSN: |
1477-9137
0021-9533 |
| DOI: | 10.1242/jcs.096818 |