Programmed Delay of a Virulence Circuit Promotes Salmonella Pathogenicity

Gespeichert in:

Bibliographische Detailangaben
Zeitschriftentitel:	mBio
Personen und Körperschaften:	Choi, Jeongjoon, Kim, Heeju, Chang, Yoonjee, Yoo, Woongjae, Kim, Dajeong, Ryu, Sangryeol
In:	mBio, 10, 2019, 2
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Virology Microbiology

Details
Zusammenfassung:	<jats:p> To accomplish successful infection, pathogens must operate their virulence programs in a precise, time-sensitive, and coordinated manner. A major question is how pathogens control the timing of virulence gene expression during infection. Here we report that the intracellular pathogen <jats:italic>Salmonella</jats:italic> controls the timing and level of virulence gene expression by using an inhibitory protein, EIIA <jats:sup>Ntr</jats:sup> . A DNA binding master virulence regulator, PhoP, controls various virulence genes inside acidic phagosomes. <jats:italic>Salmonella</jats:italic> decreases EIIA <jats:sup>Ntr</jats:sup> amounts at acidic pH in a Lon- and PhoP-dependent manner. This, in turn, promotes expression of the PhoP-activated virulence program because EIIA <jats:sup>Ntr</jats:sup> hampers activation of PhoP-regulated genes by interfering with PhoP binding to DNA. EIIA <jats:sup>Ntr</jats:sup> enables <jats:italic>Salmonella</jats:italic> to impede the activation of PhoP-regulated gene expression inside macrophages. Our findings suggest that <jats:italic>Salmonella</jats:italic> achieves programmed delay of virulence gene activation by adjusting levels of an inhibitory factor. </jats:p>
ISSN:	2161-2129 2150-7511
DOI:	10.1128/mbio.00291-19