Bovine Viral Diarrhea Virus Entry Is Dependent on Clathrin-Mediated Endocytosis

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Bibliographische Detailangaben
Zeitschriftentitel:	Journal of Virology
Personen und Körperschaften:	Lecot, Steve, Belouzard, Sandrine, Dubuisson, Jean, Rouillé, Yves
In:	Journal of Virology, 79, 2005, 16, S. 10826-10829
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Virology Insect Science Immunology Microbiology

Details
Zusammenfassung:	<jats:title>ABSTRACT</jats:title> <jats:p>Cellular mechanisms of bovine viral diarrhea virus (BVDV) entry in MDBK cells were investigated. Chloroquine, bafilomycin A1, or ammonium chloride inhibited BVDV infection, indicating that an acidic endosomal pH is required for BVDV entry. The tyrosine kinase inhibitor genistein partially inhibited BVDV infection at a postentry step, whereas BVDV entry was strongly inhibited by chlorpromazine or by the overexpression of a dominant-negative form of EPS15, a protein essential for the formation of clathrin-coated vesicles at the plasma membrane. Together, these data indicate that BVDV infection requires an active clathrin-dependent endocytic pathway.</jats:p>
Umfang:	10826-10829
ISSN:	0022-538X 1098-5514
DOI:	10.1128/jvi.79.16.10826-10829.2005