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Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 187, 2005, 11, S. 3698-3707 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Watanabe, S. Ito, T. Takeuchi, F. Endo, M. Okuno, E. Hiramatsu, K. Watanabe, S. Ito, T. Takeuchi, F. Endo, M. Okuno, E. Hiramatsu, K. |
|---|---|
| author |
Watanabe, S. Ito, T. Takeuchi, F. Endo, M. Okuno, E. Hiramatsu, K. |
| spellingShingle |
Watanabe, S. Ito, T. Takeuchi, F. Endo, M. Okuno, E. Hiramatsu, K. Journal of Bacteriology Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus Molecular Biology Microbiology |
| author_sort |
watanabe, s. |
| spelling |
Watanabe, S. Ito, T. Takeuchi, F. Endo, M. Okuno, E. Hiramatsu, K. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.187.11.3698-3707.2005 <jats:title>ABSTRACT</jats:title> <jats:p> Staphylocoagulase detection is the hallmark of a <jats:italic>Staphylococcus aureus</jats:italic> infection. Ten different serotypes of staphylocoagulases have been reported to date. We determined the nucleotide sequences of seven staphylocoagulase genes ( <jats:italic>coa</jats:italic> ) and their surrounding regions to compare structures of all 10 staphylocoagulase serotypes, and we inferred their derivations. We found that all staphylocoagulases are comprised of six regions: signal sequence, D1 region, D2 region, central region, repeat region, and C-terminal sequence. Amino acids at both ends, 33 amino acids in the N terminal (the signal sequences and the seven N-terminal amino acids in the D1 region) and 5 amino acids in the C terminal, were exactly identical among the 10 serotypes. The central regions were conserved with identities between 80.6 and 94.1% and similarities between 82.8 and 94.6%. Repeat regions comprising tandem repeats of 27 amino acids with a 92% identity on average were polymorphic in the number of repeats. On the other hand, D1 regions other than the seven N-terminal amino acids and D2 regions were less homologous, with diverged identities from 41.5 to 84.5% and 47.0 to 88.9%, respectively, and similarities from 53.5 to 88.7% and 56.8 to 91.9%, respectively, although the predicted prothrombin-binding sites were conserved among them. In contrast, flanking regions of <jats:italic>coa</jats:italic> were highly homologous, with nucleotide identities of more than 97.1%. Phylogenetic relations among <jats:italic>coa</jats:italic> did not correlate with those among the flanking regions or housekeeping genes used for multilocus sequence typing. These data indicate that <jats:italic>coa</jats:italic> could be transmitted to <jats:italic>S. aureus</jats:italic> , while the less homologous regions in <jats:italic>coa</jats:italic> presumed to be responsible for different antigenicities might have evolved independently. </jats:p> Structural Comparison of Ten Serotypes of Staphylocoagulases in <i>Staphylococcus aureus</i> Journal of Bacteriology |
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| title |
Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_unstemmed |
Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_full |
Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_fullStr |
Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_full_unstemmed |
Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_short |
Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_sort |
structural comparison of ten serotypes of staphylocoagulases in
<i>staphylococcus aureus</i> |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.187.11.3698-3707.2005 |
| publishDate |
2005 |
| physical |
3698-3707 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Staphylocoagulase detection is the hallmark of a
<jats:italic>Staphylococcus aureus</jats:italic>
infection. Ten different serotypes of staphylocoagulases have been reported to date. We determined the nucleotide sequences of seven staphylocoagulase genes (
<jats:italic>coa</jats:italic>
) and their surrounding regions to compare structures of all 10 staphylocoagulase serotypes, and we inferred their derivations. We found that all staphylocoagulases are comprised of six regions: signal sequence, D1 region, D2 region, central region, repeat region, and C-terminal sequence. Amino acids at both ends, 33 amino acids in the N terminal (the signal sequences and the seven N-terminal amino acids in the D1 region) and 5 amino acids in the C terminal, were exactly identical among the 10 serotypes. The central regions were conserved with identities between 80.6 and 94.1% and similarities between 82.8 and 94.6%. Repeat regions comprising tandem repeats of 27 amino acids with a 92% identity on average were polymorphic in the number of repeats. On the other hand, D1 regions other than the seven N-terminal amino acids and D2 regions were less homologous, with diverged identities from 41.5 to 84.5% and 47.0 to 88.9%, respectively, and similarities from 53.5 to 88.7% and 56.8 to 91.9%, respectively, although the predicted prothrombin-binding sites were conserved among them. In contrast, flanking regions of
<jats:italic>coa</jats:italic>
were highly homologous, with nucleotide identities of more than 97.1%. Phylogenetic relations among
<jats:italic>coa</jats:italic>
did not correlate with those among the flanking regions or housekeeping genes used for multilocus sequence typing. These data indicate that
<jats:italic>coa</jats:italic>
could be transmitted to
<jats:italic>S. aureus</jats:italic>
, while the less homologous regions in
<jats:italic>coa</jats:italic>
presumed to be responsible for different antigenicities might have evolved independently.
</jats:p> |
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| author | Watanabe, S., Ito, T., Takeuchi, F., Endo, M., Okuno, E., Hiramatsu, K. |
| author_facet | Watanabe, S., Ito, T., Takeuchi, F., Endo, M., Okuno, E., Hiramatsu, K., Watanabe, S., Ito, T., Takeuchi, F., Endo, M., Okuno, E., Hiramatsu, K. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Staphylocoagulase detection is the hallmark of a <jats:italic>Staphylococcus aureus</jats:italic> infection. Ten different serotypes of staphylocoagulases have been reported to date. We determined the nucleotide sequences of seven staphylocoagulase genes ( <jats:italic>coa</jats:italic> ) and their surrounding regions to compare structures of all 10 staphylocoagulase serotypes, and we inferred their derivations. We found that all staphylocoagulases are comprised of six regions: signal sequence, D1 region, D2 region, central region, repeat region, and C-terminal sequence. Amino acids at both ends, 33 amino acids in the N terminal (the signal sequences and the seven N-terminal amino acids in the D1 region) and 5 amino acids in the C terminal, were exactly identical among the 10 serotypes. The central regions were conserved with identities between 80.6 and 94.1% and similarities between 82.8 and 94.6%. Repeat regions comprising tandem repeats of 27 amino acids with a 92% identity on average were polymorphic in the number of repeats. On the other hand, D1 regions other than the seven N-terminal amino acids and D2 regions were less homologous, with diverged identities from 41.5 to 84.5% and 47.0 to 88.9%, respectively, and similarities from 53.5 to 88.7% and 56.8 to 91.9%, respectively, although the predicted prothrombin-binding sites were conserved among them. In contrast, flanking regions of <jats:italic>coa</jats:italic> were highly homologous, with nucleotide identities of more than 97.1%. Phylogenetic relations among <jats:italic>coa</jats:italic> did not correlate with those among the flanking regions or housekeeping genes used for multilocus sequence typing. These data indicate that <jats:italic>coa</jats:italic> could be transmitted to <jats:italic>S. aureus</jats:italic> , while the less homologous regions in <jats:italic>coa</jats:italic> presumed to be responsible for different antigenicities might have evolved independently. </jats:p> |
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| spelling | Watanabe, S. Ito, T. Takeuchi, F. Endo, M. Okuno, E. Hiramatsu, K. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.187.11.3698-3707.2005 <jats:title>ABSTRACT</jats:title> <jats:p> Staphylocoagulase detection is the hallmark of a <jats:italic>Staphylococcus aureus</jats:italic> infection. Ten different serotypes of staphylocoagulases have been reported to date. We determined the nucleotide sequences of seven staphylocoagulase genes ( <jats:italic>coa</jats:italic> ) and their surrounding regions to compare structures of all 10 staphylocoagulase serotypes, and we inferred their derivations. We found that all staphylocoagulases are comprised of six regions: signal sequence, D1 region, D2 region, central region, repeat region, and C-terminal sequence. Amino acids at both ends, 33 amino acids in the N terminal (the signal sequences and the seven N-terminal amino acids in the D1 region) and 5 amino acids in the C terminal, were exactly identical among the 10 serotypes. The central regions were conserved with identities between 80.6 and 94.1% and similarities between 82.8 and 94.6%. Repeat regions comprising tandem repeats of 27 amino acids with a 92% identity on average were polymorphic in the number of repeats. On the other hand, D1 regions other than the seven N-terminal amino acids and D2 regions were less homologous, with diverged identities from 41.5 to 84.5% and 47.0 to 88.9%, respectively, and similarities from 53.5 to 88.7% and 56.8 to 91.9%, respectively, although the predicted prothrombin-binding sites were conserved among them. In contrast, flanking regions of <jats:italic>coa</jats:italic> were highly homologous, with nucleotide identities of more than 97.1%. Phylogenetic relations among <jats:italic>coa</jats:italic> did not correlate with those among the flanking regions or housekeeping genes used for multilocus sequence typing. These data indicate that <jats:italic>coa</jats:italic> could be transmitted to <jats:italic>S. aureus</jats:italic> , while the less homologous regions in <jats:italic>coa</jats:italic> presumed to be responsible for different antigenicities might have evolved independently. </jats:p> Structural Comparison of Ten Serotypes of Staphylocoagulases in <i>Staphylococcus aureus</i> Journal of Bacteriology |
| spellingShingle | Watanabe, S., Ito, T., Takeuchi, F., Endo, M., Okuno, E., Hiramatsu, K., Journal of Bacteriology, Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus, Molecular Biology, Microbiology |
| title | Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_full | Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_fullStr | Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_full_unstemmed | Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_short | Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| title_sort | structural comparison of ten serotypes of staphylocoagulases in <i>staphylococcus aureus</i> |
| title_unstemmed | Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.187.11.3698-3707.2005 |