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Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus
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| Zeitschriftentitel: | Journal of Bacteriology |
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| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 187, 2005, 11, S. 3698-3707 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> Staphylocoagulase detection is the hallmark of a <jats:italic>Staphylococcus aureus</jats:italic> infection. Ten different serotypes of staphylocoagulases have been reported to date. We determined the nucleotide sequences of seven staphylocoagulase genes ( <jats:italic>coa</jats:italic> ) and their surrounding regions to compare structures of all 10 staphylocoagulase serotypes, and we inferred their derivations. We found that all staphylocoagulases are comprised of six regions: signal sequence, D1 region, D2 region, central region, repeat region, and C-terminal sequence. Amino acids at both ends, 33 amino acids in the N terminal (the signal sequences and the seven N-terminal amino acids in the D1 region) and 5 amino acids in the C terminal, were exactly identical among the 10 serotypes. The central regions were conserved with identities between 80.6 and 94.1% and similarities between 82.8 and 94.6%. Repeat regions comprising tandem repeats of 27 amino acids with a 92% identity on average were polymorphic in the number of repeats. On the other hand, D1 regions other than the seven N-terminal amino acids and D2 regions were less homologous, with diverged identities from 41.5 to 84.5% and 47.0 to 88.9%, respectively, and similarities from 53.5 to 88.7% and 56.8 to 91.9%, respectively, although the predicted prothrombin-binding sites were conserved among them. In contrast, flanking regions of <jats:italic>coa</jats:italic> were highly homologous, with nucleotide identities of more than 97.1%. Phylogenetic relations among <jats:italic>coa</jats:italic> did not correlate with those among the flanking regions or housekeeping genes used for multilocus sequence typing. These data indicate that <jats:italic>coa</jats:italic> could be transmitted to <jats:italic>S. aureus</jats:italic> , while the less homologous regions in <jats:italic>coa</jats:italic> presumed to be responsible for different antigenicities might have evolved independently. </jats:p> |
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| Umfang: | 3698-3707 |
| ISSN: |
1098-5530
0021-9193 |
| DOI: | 10.1128/jb.187.11.3698-3707.2005 |