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Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Bacteriology, 181, 1999, 2, S. 418-425 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Promadej, N. Fiedler, F. Cossart, P. Dramsi, S. Kathariou, S. Promadej, N. Fiedler, F. Cossart, P. Dramsi, S. Kathariou, S. |
|---|---|
| author |
Promadej, N. Fiedler, F. Cossart, P. Dramsi, S. Kathariou, S. |
| spellingShingle |
Promadej, N. Fiedler, F. Cossart, P. Dramsi, S. Kathariou, S. Journal of Bacteriology Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene Molecular Biology Microbiology |
| author_sort |
promadej, n. |
| spelling |
Promadej, N. Fiedler, F. Cossart, P. Dramsi, S. Kathariou, S. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.181.2.418-425.1999 <jats:title>ABSTRACT</jats:title> <jats:p> We have identified a novel gene, <jats:italic>gtcA</jats:italic> , involved in the decoration of cell wall teichoic acid of <jats:italic>Listeria monocytogenes</jats:italic> serotype 4b with galactose and glucose. Insertional inactivation of <jats:italic>gtcA</jats:italic> brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. Complementation of the mutants with the cloned <jats:italic>gtcA</jats:italic> in <jats:italic>trans</jats:italic> restored galactose and glucose on teichoic acid to wild-type levels. The complemented strains also recovered reactivity with c74.22. Within <jats:italic>L. monocytogenes</jats:italic> , sequences homologous to <jats:italic>gtcA</jats:italic> were found in all serogroup 4 isolates but not in strains of any other serotypes. In serotype 4b, <jats:italic>gtcA</jats:italic> appears to be the first member of a bicistronic operon which includes a gene with homology to <jats:italic>Bacillus subtilis rpmE</jats:italic> , encoding ribosomal protein L31. In contrast to <jats:italic>gtcA</jats:italic> , the latter gene appears conserved among all screened serotypes of <jats:italic>L. monocytogenes</jats:italic> . </jats:p> Cell Wall Teichoic Acid Glycosylation in <i>Listeria monocytogenes</i> Serotype 4b Requires <i>gtcA</i> , a Novel, Serogroup-Specific Gene Journal of Bacteriology |
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10.1128/jb.181.2.418-425.1999 |
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Biologie |
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American Society for Microbiology, 1999 |
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American Society for Microbiology, 1999 |
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0021-9193 1098-5530 |
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| title |
Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_unstemmed |
Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_full |
Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_fullStr |
Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_full_unstemmed |
Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_short |
Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_sort |
cell wall teichoic acid glycosylation in
<i>listeria monocytogenes</i>
serotype 4b requires
<i>gtca</i>
, a novel, serogroup-specific gene |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.181.2.418-425.1999 |
| publishDate |
1999 |
| physical |
418-425 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
We have identified a novel gene,
<jats:italic>gtcA</jats:italic>
, involved in the decoration of cell wall teichoic acid of
<jats:italic>Listeria monocytogenes</jats:italic>
serotype 4b with galactose and glucose. Insertional inactivation of
<jats:italic>gtcA</jats:italic>
brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. Complementation of the mutants with the cloned
<jats:italic>gtcA</jats:italic>
in
<jats:italic>trans</jats:italic>
restored galactose and glucose on teichoic acid to wild-type levels. The complemented strains also recovered reactivity with c74.22. Within
<jats:italic>L. monocytogenes</jats:italic>
, sequences homologous to
<jats:italic>gtcA</jats:italic>
were found in all serogroup 4 isolates but not in strains of any other serotypes. In serotype 4b,
<jats:italic>gtcA</jats:italic>
appears to be the first member of a bicistronic operon which includes a gene with homology to
<jats:italic>Bacillus subtilis rpmE</jats:italic>
, encoding ribosomal protein L31. In contrast to
<jats:italic>gtcA</jats:italic>
, the latter gene appears conserved among all screened serotypes of
<jats:italic>L. monocytogenes</jats:italic>
.
</jats:p> |
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| author | Promadej, N., Fiedler, F., Cossart, P., Dramsi, S., Kathariou, S. |
| author_facet | Promadej, N., Fiedler, F., Cossart, P., Dramsi, S., Kathariou, S., Promadej, N., Fiedler, F., Cossart, P., Dramsi, S., Kathariou, S. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> We have identified a novel gene, <jats:italic>gtcA</jats:italic> , involved in the decoration of cell wall teichoic acid of <jats:italic>Listeria monocytogenes</jats:italic> serotype 4b with galactose and glucose. Insertional inactivation of <jats:italic>gtcA</jats:italic> brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. Complementation of the mutants with the cloned <jats:italic>gtcA</jats:italic> in <jats:italic>trans</jats:italic> restored galactose and glucose on teichoic acid to wild-type levels. The complemented strains also recovered reactivity with c74.22. Within <jats:italic>L. monocytogenes</jats:italic> , sequences homologous to <jats:italic>gtcA</jats:italic> were found in all serogroup 4 isolates but not in strains of any other serotypes. In serotype 4b, <jats:italic>gtcA</jats:italic> appears to be the first member of a bicistronic operon which includes a gene with homology to <jats:italic>Bacillus subtilis rpmE</jats:italic> , encoding ribosomal protein L31. In contrast to <jats:italic>gtcA</jats:italic> , the latter gene appears conserved among all screened serotypes of <jats:italic>L. monocytogenes</jats:italic> . </jats:p> |
| doi_str_mv | 10.1128/jb.181.2.418-425.1999 |
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| imprint | American Society for Microbiology, 1999 |
| imprint_str_mv | American Society for Microbiology, 1999 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
| issn | 0021-9193, 1098-5530 |
| issn_str_mv | 0021-9193, 1098-5530 |
| language | English |
| last_indexed | 2024-03-01T17:07:21.492Z |
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| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 418-425 |
| publishDate | 1999 |
| publishDateSort | 1999 |
| publisher | American Society for Microbiology |
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| series | Journal of Bacteriology |
| source_id | 49 |
| spelling | Promadej, N. Fiedler, F. Cossart, P. Dramsi, S. Kathariou, S. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.181.2.418-425.1999 <jats:title>ABSTRACT</jats:title> <jats:p> We have identified a novel gene, <jats:italic>gtcA</jats:italic> , involved in the decoration of cell wall teichoic acid of <jats:italic>Listeria monocytogenes</jats:italic> serotype 4b with galactose and glucose. Insertional inactivation of <jats:italic>gtcA</jats:italic> brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. Complementation of the mutants with the cloned <jats:italic>gtcA</jats:italic> in <jats:italic>trans</jats:italic> restored galactose and glucose on teichoic acid to wild-type levels. The complemented strains also recovered reactivity with c74.22. Within <jats:italic>L. monocytogenes</jats:italic> , sequences homologous to <jats:italic>gtcA</jats:italic> were found in all serogroup 4 isolates but not in strains of any other serotypes. In serotype 4b, <jats:italic>gtcA</jats:italic> appears to be the first member of a bicistronic operon which includes a gene with homology to <jats:italic>Bacillus subtilis rpmE</jats:italic> , encoding ribosomal protein L31. In contrast to <jats:italic>gtcA</jats:italic> , the latter gene appears conserved among all screened serotypes of <jats:italic>L. monocytogenes</jats:italic> . </jats:p> Cell Wall Teichoic Acid Glycosylation in <i>Listeria monocytogenes</i> Serotype 4b Requires <i>gtcA</i> , a Novel, Serogroup-Specific Gene Journal of Bacteriology |
| spellingShingle | Promadej, N., Fiedler, F., Cossart, P., Dramsi, S., Kathariou, S., Journal of Bacteriology, Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene, Molecular Biology, Microbiology |
| title | Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_full | Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_fullStr | Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_full_unstemmed | Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_short | Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| title_sort | cell wall teichoic acid glycosylation in <i>listeria monocytogenes</i> serotype 4b requires <i>gtca</i> , a novel, serogroup-specific gene |
| title_unstemmed | Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.181.2.418-425.1999 |