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Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene
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| Zeitschriftentitel: | Journal of Bacteriology |
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| Personen und Körperschaften: | , , , , |
| In: | Journal of Bacteriology, 181, 1999, 2, S. 418-425 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> We have identified a novel gene, <jats:italic>gtcA</jats:italic> , involved in the decoration of cell wall teichoic acid of <jats:italic>Listeria monocytogenes</jats:italic> serotype 4b with galactose and glucose. Insertional inactivation of <jats:italic>gtcA</jats:italic> brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. Complementation of the mutants with the cloned <jats:italic>gtcA</jats:italic> in <jats:italic>trans</jats:italic> restored galactose and glucose on teichoic acid to wild-type levels. The complemented strains also recovered reactivity with c74.22. Within <jats:italic>L. monocytogenes</jats:italic> , sequences homologous to <jats:italic>gtcA</jats:italic> were found in all serogroup 4 isolates but not in strains of any other serotypes. In serotype 4b, <jats:italic>gtcA</jats:italic> appears to be the first member of a bicistronic operon which includes a gene with homology to <jats:italic>Bacillus subtilis rpmE</jats:italic> , encoding ribosomal protein L31. In contrast to <jats:italic>gtcA</jats:italic> , the latter gene appears conserved among all screened serotypes of <jats:italic>L. monocytogenes</jats:italic> . </jats:p> |
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| Umfang: | 418-425 |
| ISSN: |
0021-9193
1098-5530 |
| DOI: | 10.1128/jb.181.2.418-425.1999 |