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Evolutionary relationships among extradiol dioxygenases
Gespeichert in:
| Zeitschriftentitel: | Journal of Bacteriology |
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| Personen und Körperschaften: | , |
| In: | Journal of Bacteriology, 178, 1996, 20, S. 5930-5937 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:p>A structure-validated alignment of 35 extradiol dioxygenase sequences including two-domain and one-domain enzymes was derived. Strictly conserved residues include the metal ion ligands and several catalytically essential active site residues, as well as a number of structurally important residues that are remote from the active site. Phylogenetic analyses based on this alignment indicate that the ancestral extradiol dioxygenase was a one-domain enzyme and that the two-domain enzymes arose from a single genetic duplication event. Subsequent divergence among the two-domain dioxygenases has resulted in several families, two of which are based on substrate preference. In several cases, the two domains of a given enzyme express different phylogenies, suggesting the possibility that such enzymes arose from the recombination of genes encoding different dioxygenases. A phylogeny-based classification system for extradiol dioxygenases is proposed.</jats:p> |
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| Umfang: | 5930-5937 |
| ISSN: |
0021-9193
1098-5530 |
| DOI: | 10.1128/jb.178.20.5930-5937.1996 |