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The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Bacteriology, 195, 2013, 20, S. 4600-4610 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Amari, Diana T. Marques, Cláudia N. H. Davies, David G. Amari, Diana T. Marques, Cláudia N. H. Davies, David G. |
|---|---|
| author |
Amari, Diana T. Marques, Cláudia N. H. Davies, David G. |
| spellingShingle |
Amari, Diana T. Marques, Cláudia N. H. Davies, David G. Journal of Bacteriology The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid Molecular Biology Microbiology |
| author_sort |
amari, diana t. |
| spelling |
Amari, Diana T. Marques, Cláudia N. H. Davies, David G. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00707-13 <jats:title>ABSTRACT</jats:title> <jats:p> In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer <jats:italic>cis</jats:italic> -2-decenoic acid in the human pathogen <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Pseudomonas aeruginosa</jats:named-content> . The protein is encoded by PA14_54640 (PA0745), named <jats:italic>dspI</jats:italic> for <jats:italic>d</jats:italic> i <jats:italic>sp</jats:italic> ersion <jats:italic>i</jats:italic> nducer. The gene sequence for this protein shows significant homology to RpfF in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Xanthomonas campestris</jats:named-content> . Inactivation of <jats:italic>dspI</jats:italic> was shown to abolish biofilm dispersion autoinduction in continuous cultures of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. aeruginosa</jats:named-content> and resulted in biofilms that were significantly greater in thickness and biomass than those of the parental wild-type strain. Dispersion was shown to be inducible in <jats:italic>dspI</jats:italic> mutants by the exogenous addition of synthetic <jats:italic>cis</jats:italic> -2-decenoic acid or by complementation of Δ <jats:italic>dspI in trans</jats:italic> under the control of an arabinose-inducible promoter. Mutation of <jats:italic>dspI</jats:italic> was also shown to abolish <jats:italic>cis</jats:italic> -2-decenoic acid production, as revealed by gas chromatography-mass spectrometry (GC-MS) analysis of cell-free spent culture medium. The transcript abundance of <jats:italic>dspI</jats:italic> correlated with cell density, as determined by quantitative reverse transcriptase (RT) PCR. This regulation is consistent with the characterization of <jats:italic>cis</jats:italic> -2-decenoic acid as a cell-to-cell communication molecule that regulates biofilm dispersion in a cell density-dependent manner. </jats:p> The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer <i>cis</i> -2-Decenoic Acid Journal of Bacteriology |
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10.1128/jb.00707-13 |
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American Society for Microbiology, 2013 |
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American Society for Microbiology, 2013 |
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0021-9193 1098-5530 |
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2013 |
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Journal of Bacteriology |
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| title |
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_unstemmed |
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_full |
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_fullStr |
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_full_unstemmed |
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_short |
The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_sort |
the putative enoyl-coenzyme a hydratase dspi is required for production of the pseudomonas aeruginosa biofilm dispersion autoinducer
<i>cis</i>
-2-decenoic acid |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.00707-13 |
| publishDate |
2013 |
| physical |
4600-4610 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer
<jats:italic>cis</jats:italic>
-2-decenoic acid in the human pathogen
<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Pseudomonas aeruginosa</jats:named-content>
. The protein is encoded by PA14_54640 (PA0745), named
<jats:italic>dspI</jats:italic>
for
<jats:italic>d</jats:italic>
i
<jats:italic>sp</jats:italic>
ersion
<jats:italic>i</jats:italic>
nducer. The gene sequence for this protein shows significant homology to RpfF in
<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Xanthomonas campestris</jats:named-content>
. Inactivation of
<jats:italic>dspI</jats:italic>
was shown to abolish biofilm dispersion autoinduction in continuous cultures of
<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. aeruginosa</jats:named-content>
and resulted in biofilms that were significantly greater in thickness and biomass than those of the parental wild-type strain. Dispersion was shown to be inducible in
<jats:italic>dspI</jats:italic>
mutants by the exogenous addition of synthetic
<jats:italic>cis</jats:italic>
-2-decenoic acid or by complementation of Δ
<jats:italic>dspI in trans</jats:italic>
under the control of an arabinose-inducible promoter. Mutation of
<jats:italic>dspI</jats:italic>
was also shown to abolish
<jats:italic>cis</jats:italic>
-2-decenoic acid production, as revealed by gas chromatography-mass spectrometry (GC-MS) analysis of cell-free spent culture medium. The transcript abundance of
<jats:italic>dspI</jats:italic>
correlated with cell density, as determined by quantitative reverse transcriptase (RT) PCR. This regulation is consistent with the characterization of
<jats:italic>cis</jats:italic>
-2-decenoic acid as a cell-to-cell communication molecule that regulates biofilm dispersion in a cell density-dependent manner.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer <jats:italic>cis</jats:italic> -2-decenoic acid in the human pathogen <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Pseudomonas aeruginosa</jats:named-content> . The protein is encoded by PA14_54640 (PA0745), named <jats:italic>dspI</jats:italic> for <jats:italic>d</jats:italic> i <jats:italic>sp</jats:italic> ersion <jats:italic>i</jats:italic> nducer. The gene sequence for this protein shows significant homology to RpfF in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Xanthomonas campestris</jats:named-content> . Inactivation of <jats:italic>dspI</jats:italic> was shown to abolish biofilm dispersion autoinduction in continuous cultures of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. aeruginosa</jats:named-content> and resulted in biofilms that were significantly greater in thickness and biomass than those of the parental wild-type strain. Dispersion was shown to be inducible in <jats:italic>dspI</jats:italic> mutants by the exogenous addition of synthetic <jats:italic>cis</jats:italic> -2-decenoic acid or by complementation of Δ <jats:italic>dspI in trans</jats:italic> under the control of an arabinose-inducible promoter. Mutation of <jats:italic>dspI</jats:italic> was also shown to abolish <jats:italic>cis</jats:italic> -2-decenoic acid production, as revealed by gas chromatography-mass spectrometry (GC-MS) analysis of cell-free spent culture medium. The transcript abundance of <jats:italic>dspI</jats:italic> correlated with cell density, as determined by quantitative reverse transcriptase (RT) PCR. This regulation is consistent with the characterization of <jats:italic>cis</jats:italic> -2-decenoic acid as a cell-to-cell communication molecule that regulates biofilm dispersion in a cell density-dependent manner. </jats:p> |
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| spelling | Amari, Diana T. Marques, Cláudia N. H. Davies, David G. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00707-13 <jats:title>ABSTRACT</jats:title> <jats:p> In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer <jats:italic>cis</jats:italic> -2-decenoic acid in the human pathogen <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Pseudomonas aeruginosa</jats:named-content> . The protein is encoded by PA14_54640 (PA0745), named <jats:italic>dspI</jats:italic> for <jats:italic>d</jats:italic> i <jats:italic>sp</jats:italic> ersion <jats:italic>i</jats:italic> nducer. The gene sequence for this protein shows significant homology to RpfF in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Xanthomonas campestris</jats:named-content> . Inactivation of <jats:italic>dspI</jats:italic> was shown to abolish biofilm dispersion autoinduction in continuous cultures of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. aeruginosa</jats:named-content> and resulted in biofilms that were significantly greater in thickness and biomass than those of the parental wild-type strain. Dispersion was shown to be inducible in <jats:italic>dspI</jats:italic> mutants by the exogenous addition of synthetic <jats:italic>cis</jats:italic> -2-decenoic acid or by complementation of Δ <jats:italic>dspI in trans</jats:italic> under the control of an arabinose-inducible promoter. Mutation of <jats:italic>dspI</jats:italic> was also shown to abolish <jats:italic>cis</jats:italic> -2-decenoic acid production, as revealed by gas chromatography-mass spectrometry (GC-MS) analysis of cell-free spent culture medium. The transcript abundance of <jats:italic>dspI</jats:italic> correlated with cell density, as determined by quantitative reverse transcriptase (RT) PCR. This regulation is consistent with the characterization of <jats:italic>cis</jats:italic> -2-decenoic acid as a cell-to-cell communication molecule that regulates biofilm dispersion in a cell density-dependent manner. </jats:p> The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer <i>cis</i> -2-Decenoic Acid Journal of Bacteriology |
| spellingShingle | Amari, Diana T., Marques, Cláudia N. H., Davies, David G., Journal of Bacteriology, The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid, Molecular Biology, Microbiology |
| title | The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_full | The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_fullStr | The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_full_unstemmed | The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_short | The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| title_sort | the putative enoyl-coenzyme a hydratase dspi is required for production of the pseudomonas aeruginosa biofilm dispersion autoinducer <i>cis</i> -2-decenoic acid |
| title_unstemmed | The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.00707-13 |