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The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid
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| Zeitschriftentitel: | Journal of Bacteriology |
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| Personen und Körperschaften: | , , |
| In: | Journal of Bacteriology, 195, 2013, 20, S. 4600-4610 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer <jats:italic>cis</jats:italic> -2-decenoic acid in the human pathogen <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Pseudomonas aeruginosa</jats:named-content> . The protein is encoded by PA14_54640 (PA0745), named <jats:italic>dspI</jats:italic> for <jats:italic>d</jats:italic> i <jats:italic>sp</jats:italic> ersion <jats:italic>i</jats:italic> nducer. The gene sequence for this protein shows significant homology to RpfF in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Xanthomonas campestris</jats:named-content> . Inactivation of <jats:italic>dspI</jats:italic> was shown to abolish biofilm dispersion autoinduction in continuous cultures of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">P. aeruginosa</jats:named-content> and resulted in biofilms that were significantly greater in thickness and biomass than those of the parental wild-type strain. Dispersion was shown to be inducible in <jats:italic>dspI</jats:italic> mutants by the exogenous addition of synthetic <jats:italic>cis</jats:italic> -2-decenoic acid or by complementation of Δ <jats:italic>dspI in trans</jats:italic> under the control of an arabinose-inducible promoter. Mutation of <jats:italic>dspI</jats:italic> was also shown to abolish <jats:italic>cis</jats:italic> -2-decenoic acid production, as revealed by gas chromatography-mass spectrometry (GC-MS) analysis of cell-free spent culture medium. The transcript abundance of <jats:italic>dspI</jats:italic> correlated with cell density, as determined by quantitative reverse transcriptase (RT) PCR. This regulation is consistent with the characterization of <jats:italic>cis</jats:italic> -2-decenoic acid as a cell-to-cell communication molecule that regulates biofilm dispersion in a cell density-dependent manner. </jats:p> |
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| Umfang: | 4600-4610 |
| ISSN: |
0021-9193
1098-5530 |
| DOI: | 10.1128/jb.00707-13 |