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A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 191, 2009, 21, S. 6612-6617 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Stagg, Robert M. Tang, Swee-Seong Carlin, Nils I. A. Talukder, Kaisar A. Cam, Phung D. Verma, Naresh K. Stagg, Robert M. Tang, Swee-Seong Carlin, Nils I. A. Talukder, Kaisar A. Cam, Phung D. Verma, Naresh K. |
|---|---|
| author |
Stagg, Robert M. Tang, Swee-Seong Carlin, Nils I. A. Talukder, Kaisar A. Cam, Phung D. Verma, Naresh K. |
| spellingShingle |
Stagg, Robert M. Tang, Swee-Seong Carlin, Nils I. A. Talukder, Kaisar A. Cam, Phung D. Verma, Naresh K. Journal of Bacteriology A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c Molecular Biology Microbiology |
| author_sort |
stagg, robert m. |
| spelling |
Stagg, Robert M. Tang, Swee-Seong Carlin, Nils I. A. Talukder, Kaisar A. Cam, Phung D. Verma, Naresh K. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00628-09 <jats:title>ABSTRACT</jats:title> <jats:p> The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized <jats:italic>gtrI</jats:italic> cluster, which is found within a cryptic prophage at the <jats:italic>proA</jats:italic> locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated <jats:italic>gtrIC</jats:italic> ) was present as part of a three-gene cluster, similar to other <jats:italic>S. flexneri</jats:italic> glucosyltransferase genes. Relative to the other <jats:italic>S. flexneri gtr</jats:italic> clusters, the <jats:italic>gtrIC</jats:italic> cluster is more distantly related and appears to have arrived in <jats:italic>S. flexneri</jats:italic> from outside the species. Analysis of surrounding sequence suggests that the <jats:italic>gtrIC</jats:italic> cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events. </jats:p> A Novel Glucosyltransferase Involved in O-Antigen Modification of <i>Shigella flexneri</i> Serotype 1c Journal of Bacteriology |
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American Society for Microbiology, 2009 |
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| title |
A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_unstemmed |
A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_full |
A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_fullStr |
A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_full_unstemmed |
A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_short |
A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_sort |
a novel glucosyltransferase involved in o-antigen modification of
<i>shigella flexneri</i>
serotype 1c |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.00628-09 |
| publishDate |
2009 |
| physical |
6612-6617 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized
<jats:italic>gtrI</jats:italic>
cluster, which is found within a cryptic prophage at the
<jats:italic>proA</jats:italic>
locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated
<jats:italic>gtrIC</jats:italic>
) was present as part of a three-gene cluster, similar to other
<jats:italic>S. flexneri</jats:italic>
glucosyltransferase genes. Relative to the other
<jats:italic>S. flexneri gtr</jats:italic>
clusters, the
<jats:italic>gtrIC</jats:italic>
cluster is more distantly related and appears to have arrived in
<jats:italic>S. flexneri</jats:italic>
from outside the species. Analysis of surrounding sequence suggests that the
<jats:italic>gtrIC</jats:italic>
cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events.
</jats:p> |
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| author | Stagg, Robert M., Tang, Swee-Seong, Carlin, Nils I. A., Talukder, Kaisar A., Cam, Phung D., Verma, Naresh K. |
| author_facet | Stagg, Robert M., Tang, Swee-Seong, Carlin, Nils I. A., Talukder, Kaisar A., Cam, Phung D., Verma, Naresh K., Stagg, Robert M., Tang, Swee-Seong, Carlin, Nils I. A., Talukder, Kaisar A., Cam, Phung D., Verma, Naresh K. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized <jats:italic>gtrI</jats:italic> cluster, which is found within a cryptic prophage at the <jats:italic>proA</jats:italic> locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated <jats:italic>gtrIC</jats:italic> ) was present as part of a three-gene cluster, similar to other <jats:italic>S. flexneri</jats:italic> glucosyltransferase genes. Relative to the other <jats:italic>S. flexneri gtr</jats:italic> clusters, the <jats:italic>gtrIC</jats:italic> cluster is more distantly related and appears to have arrived in <jats:italic>S. flexneri</jats:italic> from outside the species. Analysis of surrounding sequence suggests that the <jats:italic>gtrIC</jats:italic> cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events. </jats:p> |
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| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| spelling | Stagg, Robert M. Tang, Swee-Seong Carlin, Nils I. A. Talukder, Kaisar A. Cam, Phung D. Verma, Naresh K. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00628-09 <jats:title>ABSTRACT</jats:title> <jats:p> The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized <jats:italic>gtrI</jats:italic> cluster, which is found within a cryptic prophage at the <jats:italic>proA</jats:italic> locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated <jats:italic>gtrIC</jats:italic> ) was present as part of a three-gene cluster, similar to other <jats:italic>S. flexneri</jats:italic> glucosyltransferase genes. Relative to the other <jats:italic>S. flexneri gtr</jats:italic> clusters, the <jats:italic>gtrIC</jats:italic> cluster is more distantly related and appears to have arrived in <jats:italic>S. flexneri</jats:italic> from outside the species. Analysis of surrounding sequence suggests that the <jats:italic>gtrIC</jats:italic> cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events. </jats:p> A Novel Glucosyltransferase Involved in O-Antigen Modification of <i>Shigella flexneri</i> Serotype 1c Journal of Bacteriology |
| spellingShingle | Stagg, Robert M., Tang, Swee-Seong, Carlin, Nils I. A., Talukder, Kaisar A., Cam, Phung D., Verma, Naresh K., Journal of Bacteriology, A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c, Molecular Biology, Microbiology |
| title | A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_full | A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_fullStr | A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_full_unstemmed | A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_short | A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| title_sort | a novel glucosyltransferase involved in o-antigen modification of <i>shigella flexneri</i> serotype 1c |
| title_unstemmed | A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.00628-09 |