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A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1c
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| Zeitschriftentitel: | Journal of Bacteriology |
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| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 191, 2009, 21, S. 6612-6617 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> The O antigen of serotype 1c differs from the unmodified O antigen of serotype Y by the addition of a disaccharide (two glucosyl groups) to the tetrasaccharide repeating unit. It was shown here that addition of the first glucosyl group is mediated by the previously characterized <jats:italic>gtrI</jats:italic> cluster, which is found within a cryptic prophage at the <jats:italic>proA</jats:italic> locus in the bacterial chromosome. Transposon mutagenesis was performed to disrupt the gene responsible for addition of the second glucosyl group, causing reversion to serotype 1a. Colony immunoblotting was used to identify the desired revertants, and subsequent sequencing, cloning, and functional expression successfully identified the gene encoding serotype 1c-specific O-antigen modification. This gene (designated <jats:italic>gtrIC</jats:italic> ) was present as part of a three-gene cluster, similar to other <jats:italic>S. flexneri</jats:italic> glucosyltransferase genes. Relative to the other <jats:italic>S. flexneri gtr</jats:italic> clusters, the <jats:italic>gtrIC</jats:italic> cluster is more distantly related and appears to have arrived in <jats:italic>S. flexneri</jats:italic> from outside the species. Analysis of surrounding sequence suggests that the <jats:italic>gtrIC</jats:italic> cluster arrived via a novel bacteriophage that was subsequently rendered nonfunctional by a series of insertion events. </jats:p> |
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| Umfang: | 6612-6617 |
| ISSN: |
0021-9193
1098-5530 |
| DOI: | 10.1128/jb.00628-09 |