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In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiestera...
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 192, 2010, 11, S. 2779-2790 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Fuchs, Erin L. Brutinel, Evan D. Klem, Erich R. Fehr, Anthony R. Yahr, Timothy L. Wolfgang, Matthew C. Fuchs, Erin L. Brutinel, Evan D. Klem, Erich R. Fehr, Anthony R. Yahr, Timothy L. Wolfgang, Matthew C. |
|---|---|
| author |
Fuchs, Erin L. Brutinel, Evan D. Klem, Erich R. Fehr, Anthony R. Yahr, Timothy L. Wolfgang, Matthew C. |
| spellingShingle |
Fuchs, Erin L. Brutinel, Evan D. Klem, Erich R. Fehr, Anthony R. Yahr, Timothy L. Wolfgang, Matthew C. Journal of Bacteriology In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation Molecular Biology Microbiology |
| author_sort |
fuchs, erin l. |
| spelling |
Fuchs, Erin L. Brutinel, Evan D. Klem, Erich R. Fehr, Anthony R. Yahr, Timothy L. Wolfgang, Matthew C. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00168-10 <jats:title>ABSTRACT</jats:title> <jats:p> Cyclic AMP (cAMP) is an important second messenger signaling molecule that controls a wide variety of eukaryotic and prokaryotic responses to extracellular cues. For cAMP-dependent signaling pathways to be effective, the intracellular cAMP concentration is tightly controlled at the level of synthesis and degradation. In the opportunistic human pathogen <jats:italic>Pseudomonas aeruginosa</jats:italic> , cAMP is a key regulator of virulence gene expression. To better understand the role of cAMP homeostasis in this organism, we identified and characterized the enzyme CpdA, a putative cAMP phosphodiesterase. We demonstrate that CpdA possesses 3′,5′-cAMP phosphodiesterase activity <jats:italic>in vitro</jats:italic> and that it utilizes an iron-dependent catalytic mechanism. Deletion of <jats:italic>cpdA</jats:italic> results in the accumulation of intracellular cAMP and altered regulation of <jats:italic>P. aeruginosa</jats:italic> virulence traits. Further, we demonstrate that the cAMP-dependent transcription factor Vfr directly regulates <jats:italic>cpdA</jats:italic> expression in response to intracellular cAMP accumulation, thus providing a feedback mechanism for controlling cAMP levels and fine-tuning virulence factor expression. </jats:p> <i>In V</i> <i>itro</i> and <i>In V</i> <i>ivo</i> Characterization of the <i>Pseudomonas aeruginosa</i> Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation Journal of Bacteriology |
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| title |
In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_unstemmed |
In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_full |
In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_fullStr |
In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_full_unstemmed |
In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_short |
In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_sort |
<i>in v</i>
<i>itro</i>
and
<i>in v</i>
<i>ivo</i>
characterization of the
<i>pseudomonas aeruginosa</i>
cyclic amp (camp) phosphodiesterase cpda, required for camp homeostasis and virulence factor regulation |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.00168-10 |
| publishDate |
2010 |
| physical |
2779-2790 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Cyclic AMP (cAMP) is an important second messenger signaling molecule that controls a wide variety of eukaryotic and prokaryotic responses to extracellular cues. For cAMP-dependent signaling pathways to be effective, the intracellular cAMP concentration is tightly controlled at the level of synthesis and degradation. In the opportunistic human pathogen
<jats:italic>Pseudomonas aeruginosa</jats:italic>
, cAMP is a key regulator of virulence gene expression. To better understand the role of cAMP homeostasis in this organism, we identified and characterized the enzyme CpdA, a putative cAMP phosphodiesterase. We demonstrate that CpdA possesses 3′,5′-cAMP phosphodiesterase activity
<jats:italic>in vitro</jats:italic>
and that it utilizes an iron-dependent catalytic mechanism. Deletion of
<jats:italic>cpdA</jats:italic>
results in the accumulation of intracellular cAMP and altered regulation of
<jats:italic>P. aeruginosa</jats:italic>
virulence traits. Further, we demonstrate that the cAMP-dependent transcription factor Vfr directly regulates
<jats:italic>cpdA</jats:italic>
expression in response to intracellular cAMP accumulation, thus providing a feedback mechanism for controlling cAMP levels and fine-tuning virulence factor expression.
</jats:p> |
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| author | Fuchs, Erin L., Brutinel, Evan D., Klem, Erich R., Fehr, Anthony R., Yahr, Timothy L., Wolfgang, Matthew C. |
| author_facet | Fuchs, Erin L., Brutinel, Evan D., Klem, Erich R., Fehr, Anthony R., Yahr, Timothy L., Wolfgang, Matthew C., Fuchs, Erin L., Brutinel, Evan D., Klem, Erich R., Fehr, Anthony R., Yahr, Timothy L., Wolfgang, Matthew C. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Cyclic AMP (cAMP) is an important second messenger signaling molecule that controls a wide variety of eukaryotic and prokaryotic responses to extracellular cues. For cAMP-dependent signaling pathways to be effective, the intracellular cAMP concentration is tightly controlled at the level of synthesis and degradation. In the opportunistic human pathogen <jats:italic>Pseudomonas aeruginosa</jats:italic> , cAMP is a key regulator of virulence gene expression. To better understand the role of cAMP homeostasis in this organism, we identified and characterized the enzyme CpdA, a putative cAMP phosphodiesterase. We demonstrate that CpdA possesses 3′,5′-cAMP phosphodiesterase activity <jats:italic>in vitro</jats:italic> and that it utilizes an iron-dependent catalytic mechanism. Deletion of <jats:italic>cpdA</jats:italic> results in the accumulation of intracellular cAMP and altered regulation of <jats:italic>P. aeruginosa</jats:italic> virulence traits. Further, we demonstrate that the cAMP-dependent transcription factor Vfr directly regulates <jats:italic>cpdA</jats:italic> expression in response to intracellular cAMP accumulation, thus providing a feedback mechanism for controlling cAMP levels and fine-tuning virulence factor expression. </jats:p> |
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| spelling | Fuchs, Erin L. Brutinel, Evan D. Klem, Erich R. Fehr, Anthony R. Yahr, Timothy L. Wolfgang, Matthew C. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00168-10 <jats:title>ABSTRACT</jats:title> <jats:p> Cyclic AMP (cAMP) is an important second messenger signaling molecule that controls a wide variety of eukaryotic and prokaryotic responses to extracellular cues. For cAMP-dependent signaling pathways to be effective, the intracellular cAMP concentration is tightly controlled at the level of synthesis and degradation. In the opportunistic human pathogen <jats:italic>Pseudomonas aeruginosa</jats:italic> , cAMP is a key regulator of virulence gene expression. To better understand the role of cAMP homeostasis in this organism, we identified and characterized the enzyme CpdA, a putative cAMP phosphodiesterase. We demonstrate that CpdA possesses 3′,5′-cAMP phosphodiesterase activity <jats:italic>in vitro</jats:italic> and that it utilizes an iron-dependent catalytic mechanism. Deletion of <jats:italic>cpdA</jats:italic> results in the accumulation of intracellular cAMP and altered regulation of <jats:italic>P. aeruginosa</jats:italic> virulence traits. Further, we demonstrate that the cAMP-dependent transcription factor Vfr directly regulates <jats:italic>cpdA</jats:italic> expression in response to intracellular cAMP accumulation, thus providing a feedback mechanism for controlling cAMP levels and fine-tuning virulence factor expression. </jats:p> <i>In V</i> <i>itro</i> and <i>In V</i> <i>ivo</i> Characterization of the <i>Pseudomonas aeruginosa</i> Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation Journal of Bacteriology |
| spellingShingle | Fuchs, Erin L., Brutinel, Evan D., Klem, Erich R., Fehr, Anthony R., Yahr, Timothy L., Wolfgang, Matthew C., Journal of Bacteriology, In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation, Molecular Biology, Microbiology |
| title | In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_full | In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_fullStr | In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_full_unstemmed | In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_short | In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| title_sort | <i>in v</i> <i>itro</i> and <i>in v</i> <i>ivo</i> characterization of the <i>pseudomonas aeruginosa</i> cyclic amp (camp) phosphodiesterase cpda, required for camp homeostasis and virulence factor regulation |
| title_unstemmed | In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiesterase CpdA, Required for cAMP Homeostasis and Virulence Factor Regulation |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.00168-10 |