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In V itro and In V ivo Characterization of the Pseudomonas aeruginosa Cyclic AMP (cAMP) Phosphodiestera...
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| Zeitschriftentitel: | Journal of Bacteriology |
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| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 192, 2010, 11, S. 2779-2790 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> Cyclic AMP (cAMP) is an important second messenger signaling molecule that controls a wide variety of eukaryotic and prokaryotic responses to extracellular cues. For cAMP-dependent signaling pathways to be effective, the intracellular cAMP concentration is tightly controlled at the level of synthesis and degradation. In the opportunistic human pathogen <jats:italic>Pseudomonas aeruginosa</jats:italic> , cAMP is a key regulator of virulence gene expression. To better understand the role of cAMP homeostasis in this organism, we identified and characterized the enzyme CpdA, a putative cAMP phosphodiesterase. We demonstrate that CpdA possesses 3′,5′-cAMP phosphodiesterase activity <jats:italic>in vitro</jats:italic> and that it utilizes an iron-dependent catalytic mechanism. Deletion of <jats:italic>cpdA</jats:italic> results in the accumulation of intracellular cAMP and altered regulation of <jats:italic>P. aeruginosa</jats:italic> virulence traits. Further, we demonstrate that the cAMP-dependent transcription factor Vfr directly regulates <jats:italic>cpdA</jats:italic> expression in response to intracellular cAMP accumulation, thus providing a feedback mechanism for controlling cAMP levels and fine-tuning virulence factor expression. </jats:p> |
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| Umfang: | 2779-2790 |
| ISSN: |
0021-9193
1098-5530 |
| DOI: | 10.1128/jb.00168-10 |