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MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Journal of Clinical Microbiology, 46, 2008, 9, S. 3107-3109 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Podzorski, Raymond P. Li, Haijing Han, Jian Tang, Yi-Wei Podzorski, Raymond P. Li, Haijing Han, Jian Tang, Yi-Wei |
|---|---|
| author |
Podzorski, Raymond P. Li, Haijing Han, Jian Tang, Yi-Wei |
| spellingShingle |
Podzorski, Raymond P. Li, Haijing Han, Jian Tang, Yi-Wei Journal of Clinical Microbiology MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens Microbiology (medical) |
| author_sort |
podzorski, raymond p. |
| spelling |
Podzorski, Raymond P. Li, Haijing Han, Jian Tang, Yi-Wei 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.02347-07 <jats:title>ABSTRACT</jats:title> <jats:p> We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant <jats:italic>Staphylococcus aureus</jats:italic> (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the “gold standard,” the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% ( <jats:italic>P</jats:italic> = 0.002) and specificities of 95.8% and 98.6% ( <jats:italic>P</jats:italic> < 0.05), respectively. </jats:p> MVPlex Assay for Direct Detection of Methicillin-Resistant <i>Staphylococcus aureus</i> in Naris and Other Swab Specimens Journal of Clinical Microbiology |
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| title |
MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_unstemmed |
MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_full |
MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_fullStr |
MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_full_unstemmed |
MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_short |
MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_sort |
mvplex assay for direct detection of methicillin-resistant
<i>staphylococcus aureus</i>
in naris and other swab specimens |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.02347-07 |
| publishDate |
2008 |
| physical |
3107-3109 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant
<jats:italic>Staphylococcus aureus</jats:italic>
(MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the “gold standard,” the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% (
<jats:italic>P</jats:italic>
= 0.002) and specificities of 95.8% and 98.6% (
<jats:italic>P</jats:italic>
< 0.05), respectively.
</jats:p> |
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| author | Podzorski, Raymond P., Li, Haijing, Han, Jian, Tang, Yi-Wei |
| author_facet | Podzorski, Raymond P., Li, Haijing, Han, Jian, Tang, Yi-Wei, Podzorski, Raymond P., Li, Haijing, Han, Jian, Tang, Yi-Wei |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant <jats:italic>Staphylococcus aureus</jats:italic> (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the “gold standard,” the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% ( <jats:italic>P</jats:italic> = 0.002) and specificities of 95.8% and 98.6% ( <jats:italic>P</jats:italic> < 0.05), respectively. </jats:p> |
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| physical | 3107-3109 |
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| spelling | Podzorski, Raymond P. Li, Haijing Han, Jian Tang, Yi-Wei 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.02347-07 <jats:title>ABSTRACT</jats:title> <jats:p> We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant <jats:italic>Staphylococcus aureus</jats:italic> (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the “gold standard,” the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% ( <jats:italic>P</jats:italic> = 0.002) and specificities of 95.8% and 98.6% ( <jats:italic>P</jats:italic> < 0.05), respectively. </jats:p> MVPlex Assay for Direct Detection of Methicillin-Resistant <i>Staphylococcus aureus</i> in Naris and Other Swab Specimens Journal of Clinical Microbiology |
| spellingShingle | Podzorski, Raymond P., Li, Haijing, Han, Jian, Tang, Yi-Wei, Journal of Clinical Microbiology, MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens, Microbiology (medical) |
| title | MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_full | MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_fullStr | MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_full_unstemmed | MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_short | MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| title_sort | mvplex assay for direct detection of methicillin-resistant <i>staphylococcus aureus</i> in naris and other swab specimens |
| title_unstemmed | MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.02347-07 |