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MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
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| Personen und Körperschaften: | , , , |
| In: | Journal of Clinical Microbiology, 46, 2008, 9, S. 3107-3109 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant <jats:italic>Staphylococcus aureus</jats:italic> (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the “gold standard,” the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% ( <jats:italic>P</jats:italic> = 0.002) and specificities of 95.8% and 98.6% ( <jats:italic>P</jats:italic> < 0.05), respectively. </jats:p> |
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| Umfang: | 3107-3109 |
| ISSN: |
0095-1137
1098-660X |
| DOI: | 10.1128/jcm.02347-07 |