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Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | Applied and Environmental Microbiology, 69, 2003, 7, S. 3883-3891 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Hara-Kudo, Yukiko Sugiyama, Kanji Nishibuchi, Mitsuaki Chowdhury, Ashrafuzzaman Yatsuyanagi, Jun Ohtomo, Yoshimitsu Saito, Akinobu Nagano, Hidetoshi Nishina, Tokuhiro Nakagawa, Hiroshi Konuma, Hirotaka Miyahara, Michiko Kumagai, Susumu Hara-Kudo, Yukiko Sugiyama, Kanji Nishibuchi, Mitsuaki Chowdhury, Ashrafuzzaman Yatsuyanagi, Jun Ohtomo, Yoshimitsu Saito, Akinobu Nagano, Hidetoshi Nishina, Tokuhiro Nakagawa, Hiroshi Konuma, Hirotaka Miyahara, Michiko Kumagai, Susumu |
|---|---|
| author |
Hara-Kudo, Yukiko Sugiyama, Kanji Nishibuchi, Mitsuaki Chowdhury, Ashrafuzzaman Yatsuyanagi, Jun Ohtomo, Yoshimitsu Saito, Akinobu Nagano, Hidetoshi Nishina, Tokuhiro Nakagawa, Hiroshi Konuma, Hirotaka Miyahara, Michiko Kumagai, Susumu |
| spellingShingle |
Hara-Kudo, Yukiko Sugiyama, Kanji Nishibuchi, Mitsuaki Chowdhury, Ashrafuzzaman Yatsuyanagi, Jun Ohtomo, Yoshimitsu Saito, Akinobu Nagano, Hidetoshi Nishina, Tokuhiro Nakagawa, Hiroshi Konuma, Hirotaka Miyahara, Michiko Kumagai, Susumu Applied and Environmental Microbiology Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
hara-kudo, yukiko |
| spelling |
Hara-Kudo, Yukiko Sugiyama, Kanji Nishibuchi, Mitsuaki Chowdhury, Ashrafuzzaman Yatsuyanagi, Jun Ohtomo, Yoshimitsu Saito, Akinobu Nagano, Hidetoshi Nishina, Tokuhiro Nakagawa, Hiroshi Konuma, Hirotaka Miyahara, Michiko Kumagai, Susumu 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.7.3883-3891.2003 <jats:title>ABSTRACT</jats:title> <jats:p> Although thermostable direct hemolysin (TDH)-producing <jats:italic>Vibrio parahaemolyticus</jats:italic> has caused many infections in Asian countries, the United States, and other countries, it has been difficult to detect the same pathogen in seafoods and other environmental samples. In this study, we detected and enumerated <jats:italic>tdh</jats:italic> gene-positive <jats:italic>V. parahaemolyticus</jats:italic> in Japanese seafoods with a <jats:italic>tdh-</jats:italic> specific PCR method, a chromogenic agar medium, and a most-probable-number method. The <jats:italic>tdh</jats:italic> gene was detected in 33 of 329 seafood samples (10.0%). The number of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> ranged from <3 to 93/10 g. The incidence of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> tended to be high in samples contaminated with relatively high levels of total <jats:italic>V. parahaemolyticus</jats:italic> . TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were isolated from 11 of 33 <jats:italic>tdh</jats:italic> -positive samples (short-necked clam, hen clam, and rock oyster). TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were also isolated from the sediments of rivers near the coast in Japan. Representative strains of the seafood and sediment isolates were examined for the O:K serovar and by the PCR method specific to the pandemic clone and arbitrarily primed PCR and pulsed-field gel electrophoresis techniques. The results indicated that most O3:K6 <jats:italic>tdh</jats:italic> -positive strains belonged to the pandemic O3:K6 clone and suggested that serovariation took place in the Japanese environment. </jats:p> Prevalence of Pandemic Thermostable Direct Hemolysin-Producing <i>Vibrio parahaemolyticus</i> O3:K6 in Seafood and the Coastal Environment in Japan Applied and Environmental Microbiology |
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10.1128/aem.69.7.3883-3891.2003 |
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American Society for Microbiology, 2003 |
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| title |
Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_unstemmed |
Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_full |
Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_fullStr |
Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_full_unstemmed |
Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_short |
Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_sort |
prevalence of pandemic thermostable direct hemolysin-producing
<i>vibrio parahaemolyticus</i>
o3:k6 in seafood and the coastal environment in japan |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.69.7.3883-3891.2003 |
| publishDate |
2003 |
| physical |
3883-3891 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Although thermostable direct hemolysin (TDH)-producing
<jats:italic>Vibrio parahaemolyticus</jats:italic>
has caused many infections in Asian countries, the United States, and other countries, it has been difficult to detect the same pathogen in seafoods and other environmental samples. In this study, we detected and enumerated
<jats:italic>tdh</jats:italic>
gene-positive
<jats:italic>V. parahaemolyticus</jats:italic>
in Japanese seafoods with a
<jats:italic>tdh-</jats:italic>
specific PCR method, a chromogenic agar medium, and a most-probable-number method. The
<jats:italic>tdh</jats:italic>
gene was detected in 33 of 329 seafood samples (10.0%). The number of
<jats:italic>tdh</jats:italic>
-positive
<jats:italic>V. parahaemolyticus</jats:italic>
ranged from <3 to 93/10 g. The incidence of
<jats:italic>tdh</jats:italic>
-positive
<jats:italic>V. parahaemolyticus</jats:italic>
tended to be high in samples contaminated with relatively high levels of total
<jats:italic>V. parahaemolyticus</jats:italic>
. TDH-producing strains of
<jats:italic>V. parahaemolyticus</jats:italic>
were isolated from 11 of 33
<jats:italic>tdh</jats:italic>
-positive samples (short-necked clam, hen clam, and rock oyster). TDH-producing strains of
<jats:italic>V. parahaemolyticus</jats:italic>
were also isolated from the sediments of rivers near the coast in Japan. Representative strains of the seafood and sediment isolates were examined for the O:K serovar and by the PCR method specific to the pandemic clone and arbitrarily primed PCR and pulsed-field gel electrophoresis techniques. The results indicated that most O3:K6
<jats:italic>tdh</jats:italic>
-positive strains belonged to the pandemic O3:K6 clone and suggested that serovariation took place in the Japanese environment.
</jats:p> |
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| author | Hara-Kudo, Yukiko, Sugiyama, Kanji, Nishibuchi, Mitsuaki, Chowdhury, Ashrafuzzaman, Yatsuyanagi, Jun, Ohtomo, Yoshimitsu, Saito, Akinobu, Nagano, Hidetoshi, Nishina, Tokuhiro, Nakagawa, Hiroshi, Konuma, Hirotaka, Miyahara, Michiko, Kumagai, Susumu |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Although thermostable direct hemolysin (TDH)-producing <jats:italic>Vibrio parahaemolyticus</jats:italic> has caused many infections in Asian countries, the United States, and other countries, it has been difficult to detect the same pathogen in seafoods and other environmental samples. In this study, we detected and enumerated <jats:italic>tdh</jats:italic> gene-positive <jats:italic>V. parahaemolyticus</jats:italic> in Japanese seafoods with a <jats:italic>tdh-</jats:italic> specific PCR method, a chromogenic agar medium, and a most-probable-number method. The <jats:italic>tdh</jats:italic> gene was detected in 33 of 329 seafood samples (10.0%). The number of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> ranged from <3 to 93/10 g. The incidence of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> tended to be high in samples contaminated with relatively high levels of total <jats:italic>V. parahaemolyticus</jats:italic> . TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were isolated from 11 of 33 <jats:italic>tdh</jats:italic> -positive samples (short-necked clam, hen clam, and rock oyster). TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were also isolated from the sediments of rivers near the coast in Japan. Representative strains of the seafood and sediment isolates were examined for the O:K serovar and by the PCR method specific to the pandemic clone and arbitrarily primed PCR and pulsed-field gel electrophoresis techniques. The results indicated that most O3:K6 <jats:italic>tdh</jats:italic> -positive strains belonged to the pandemic O3:K6 clone and suggested that serovariation took place in the Japanese environment. </jats:p> |
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| spelling | Hara-Kudo, Yukiko Sugiyama, Kanji Nishibuchi, Mitsuaki Chowdhury, Ashrafuzzaman Yatsuyanagi, Jun Ohtomo, Yoshimitsu Saito, Akinobu Nagano, Hidetoshi Nishina, Tokuhiro Nakagawa, Hiroshi Konuma, Hirotaka Miyahara, Michiko Kumagai, Susumu 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.7.3883-3891.2003 <jats:title>ABSTRACT</jats:title> <jats:p> Although thermostable direct hemolysin (TDH)-producing <jats:italic>Vibrio parahaemolyticus</jats:italic> has caused many infections in Asian countries, the United States, and other countries, it has been difficult to detect the same pathogen in seafoods and other environmental samples. In this study, we detected and enumerated <jats:italic>tdh</jats:italic> gene-positive <jats:italic>V. parahaemolyticus</jats:italic> in Japanese seafoods with a <jats:italic>tdh-</jats:italic> specific PCR method, a chromogenic agar medium, and a most-probable-number method. The <jats:italic>tdh</jats:italic> gene was detected in 33 of 329 seafood samples (10.0%). The number of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> ranged from <3 to 93/10 g. The incidence of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> tended to be high in samples contaminated with relatively high levels of total <jats:italic>V. parahaemolyticus</jats:italic> . TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were isolated from 11 of 33 <jats:italic>tdh</jats:italic> -positive samples (short-necked clam, hen clam, and rock oyster). TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were also isolated from the sediments of rivers near the coast in Japan. Representative strains of the seafood and sediment isolates were examined for the O:K serovar and by the PCR method specific to the pandemic clone and arbitrarily primed PCR and pulsed-field gel electrophoresis techniques. The results indicated that most O3:K6 <jats:italic>tdh</jats:italic> -positive strains belonged to the pandemic O3:K6 clone and suggested that serovariation took place in the Japanese environment. </jats:p> Prevalence of Pandemic Thermostable Direct Hemolysin-Producing <i>Vibrio parahaemolyticus</i> O3:K6 in Seafood and the Coastal Environment in Japan Applied and Environmental Microbiology |
| spellingShingle | Hara-Kudo, Yukiko, Sugiyama, Kanji, Nishibuchi, Mitsuaki, Chowdhury, Ashrafuzzaman, Yatsuyanagi, Jun, Ohtomo, Yoshimitsu, Saito, Akinobu, Nagano, Hidetoshi, Nishina, Tokuhiro, Nakagawa, Hiroshi, Konuma, Hirotaka, Miyahara, Michiko, Kumagai, Susumu, Applied and Environmental Microbiology, Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_full | Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_fullStr | Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_full_unstemmed | Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_short | Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| title_sort | prevalence of pandemic thermostable direct hemolysin-producing <i>vibrio parahaemolyticus</i> o3:k6 in seafood and the coastal environment in japan |
| title_unstemmed | Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.69.7.3883-3891.2003 |