Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan

Gespeichert in:

Bibliographische Detailangaben
Zeitschriftentitel:	Applied and Environmental Microbiology
Personen und Körperschaften:	Hara-Kudo, Yukiko, Sugiyama, Kanji, Nishibuchi, Mitsuaki, Chowdhury, Ashrafuzzaman, Yatsuyanagi, Jun, Ohtomo, Yoshimitsu, Saito, Akinobu, Nagano, Hidetoshi, Nishina, Tokuhiro, Nakagawa, Hiroshi, Konuma, Hirotaka, Miyahara, Michiko, Kumagai, Susumu
In:	Applied and Environmental Microbiology, 69, 2003, 7, S. 3883-3891
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Ecology Applied Microbiology and Biotechnology Food Science Biotechnology

Details
Zusammenfassung:	<jats:title>ABSTRACT</jats:title> <jats:p> Although thermostable direct hemolysin (TDH)-producing <jats:italic>Vibrio parahaemolyticus</jats:italic> has caused many infections in Asian countries, the United States, and other countries, it has been difficult to detect the same pathogen in seafoods and other environmental samples. In this study, we detected and enumerated <jats:italic>tdh</jats:italic> gene-positive <jats:italic>V. parahaemolyticus</jats:italic> in Japanese seafoods with a <jats:italic>tdh-</jats:italic> specific PCR method, a chromogenic agar medium, and a most-probable-number method. The <jats:italic>tdh</jats:italic> gene was detected in 33 of 329 seafood samples (10.0%). The number of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> ranged from <3 to 93/10 g. The incidence of <jats:italic>tdh</jats:italic> -positive <jats:italic>V. parahaemolyticus</jats:italic> tended to be high in samples contaminated with relatively high levels of total <jats:italic>V. parahaemolyticus</jats:italic> . TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were isolated from 11 of 33 <jats:italic>tdh</jats:italic> -positive samples (short-necked clam, hen clam, and rock oyster). TDH-producing strains of <jats:italic>V. parahaemolyticus</jats:italic> were also isolated from the sediments of rivers near the coast in Japan. Representative strains of the seafood and sediment isolates were examined for the O:K serovar and by the PCR method specific to the pandemic clone and arbitrarily primed PCR and pulsed-field gel electrophoresis techniques. The results indicated that most O3:K6 <jats:italic>tdh</jats:italic> -positive strains belonged to the pandemic O3:K6 clone and suggested that serovariation took place in the Japanese environment. </jats:p>
Umfang:	3883-3891
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.69.7.3883-3891.2003