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Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Applied and Environmental Microbiology, 67, 2001, 12, S. 5420-5424 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Muilenberg, Michael Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Muilenberg, Michael |
|---|---|
| author |
Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Muilenberg, Michael |
| spellingShingle |
Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Muilenberg, Michael Applied and Environmental Microbiology Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
milton, donald k. |
| spelling |
Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Muilenberg, Michael 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.67.12.5420-5424.2001 <jats:title>ABSTRACT</jats:title> <jats:p> (1→3)-β- <jats:sc>d</jats:sc> -Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1→3)-β- <jats:sc>d</jats:sc> -glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall β- <jats:sc>d</jats:sc> -glucans as a detector reagent. The assay was highly specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans (such as that from <jats:italic>Saccharomyces</jats:italic> <jats:italic>cerevisiae</jats:italic> ) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1→3)-β- <jats:sc>d</jats:sc> -glucans in house dust samples. Metal working fluids spiked with (1→3)-β- <jats:sc>d</jats:sc> -glucans inhibited the glucan assay. Because the assay is specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules. </jats:p> Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- <scp>d</scp> -Glucan Detection in Environmental Samples Applied and Environmental Microbiology |
| doi_str_mv |
10.1128/aem.67.12.5420-5424.2001 |
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Geographie Biologie Technik Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft |
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American Society for Microbiology, 2001 |
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American Society for Microbiology, 2001 |
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0099-2240 1098-5336 |
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| title |
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_unstemmed |
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_full |
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_fullStr |
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_full_unstemmed |
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_short |
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_sort |
enzyme-linked immunosorbent assay specific for (1→6) branched, (1→3)-β-
<scp>d</scp>
-glucan detection in environmental samples |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.67.12.5420-5424.2001 |
| publishDate |
2001 |
| physical |
5420-5424 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
(1→3)-β-
<jats:sc>d</jats:sc>
-Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1→6) branched, (1→3)-β-
<jats:sc>d</jats:sc>
-glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1→3)-β-
<jats:sc>d</jats:sc>
-glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall β-
<jats:sc>d</jats:sc>
-glucans as a detector reagent. The assay was highly specific for (1→6) branched, (1→3)-β-
<jats:sc>d</jats:sc>
-glucans (such as that from
<jats:italic>Saccharomyces</jats:italic>
<jats:italic>cerevisiae</jats:italic>
) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1→3)-β-
<jats:sc>d</jats:sc>
-glucans in house dust samples. Metal working fluids spiked with (1→3)-β-
<jats:sc>d</jats:sc>
-glucans inhibited the glucan assay. Because the assay is specific for (1→6) branched, (1→3)-β-
<jats:sc>d</jats:sc>
-glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules.
</jats:p> |
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| author | Milton, Donald K., Alwis, K. Udeni, Fisette, Leslie, Muilenberg, Michael |
| author_facet | Milton, Donald K., Alwis, K. Udeni, Fisette, Leslie, Muilenberg, Michael, Milton, Donald K., Alwis, K. Udeni, Fisette, Leslie, Muilenberg, Michael |
| author_sort | milton, donald k. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> (1→3)-β- <jats:sc>d</jats:sc> -Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1→3)-β- <jats:sc>d</jats:sc> -glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall β- <jats:sc>d</jats:sc> -glucans as a detector reagent. The assay was highly specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans (such as that from <jats:italic>Saccharomyces</jats:italic> <jats:italic>cerevisiae</jats:italic> ) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1→3)-β- <jats:sc>d</jats:sc> -glucans in house dust samples. Metal working fluids spiked with (1→3)-β- <jats:sc>d</jats:sc> -glucans inhibited the glucan assay. Because the assay is specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules. </jats:p> |
| doi_str_mv | 10.1128/aem.67.12.5420-5424.2001 |
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| imprint | American Society for Microbiology, 2001 |
| imprint_str_mv | American Society for Microbiology, 2001 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0099-2240, 1098-5336 |
| issn_str_mv | 0099-2240, 1098-5336 |
| language | English |
| last_indexed | 2024-03-01T14:58:25.868Z |
| match_str | milton2001enzymelinkedimmunosorbentassayspecificfor16branched13bdglucandetectioninenvironmentalsamples |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 5420-5424 |
| publishDate | 2001 |
| publishDateSort | 2001 |
| publisher | American Society for Microbiology |
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| recordtype | ai |
| series | Applied and Environmental Microbiology |
| source_id | 49 |
| spelling | Milton, Donald K. Alwis, K. Udeni Fisette, Leslie Muilenberg, Michael 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.67.12.5420-5424.2001 <jats:title>ABSTRACT</jats:title> <jats:p> (1→3)-β- <jats:sc>d</jats:sc> -Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1→3)-β- <jats:sc>d</jats:sc> -glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall β- <jats:sc>d</jats:sc> -glucans as a detector reagent. The assay was highly specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans (such as that from <jats:italic>Saccharomyces</jats:italic> <jats:italic>cerevisiae</jats:italic> ) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1→3)-β- <jats:sc>d</jats:sc> -glucans in house dust samples. Metal working fluids spiked with (1→3)-β- <jats:sc>d</jats:sc> -glucans inhibited the glucan assay. Because the assay is specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules. </jats:p> Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- <scp>d</scp> -Glucan Detection in Environmental Samples Applied and Environmental Microbiology |
| spellingShingle | Milton, Donald K., Alwis, K. Udeni, Fisette, Leslie, Muilenberg, Michael, Applied and Environmental Microbiology, Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_full | Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_fullStr | Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_full_unstemmed | Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_short | Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| title_sort | enzyme-linked immunosorbent assay specific for (1→6) branched, (1→3)-β- <scp>d</scp> -glucan detection in environmental samples |
| title_unstemmed | Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.67.12.5420-5424.2001 |