Eintrag weiter verarbeiten
Enzyme-Linked Immunosorbent Assay Specific for (1→6) Branched, (1→3)-β- d -Glucan Detection in Environmental Samples
Gespeichert in:
| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Applied and Environmental Microbiology, 67, 2001, 12, S. 5420-5424 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> (1→3)-β- <jats:sc>d</jats:sc> -Glucans have been recognized as a potential causative agent responsible for bioaerosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1→3)-β- <jats:sc>d</jats:sc> -glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall β- <jats:sc>d</jats:sc> -glucans as a detector reagent. The assay was highly specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans (such as that from <jats:italic>Saccharomyces</jats:italic> <jats:italic>cerevisiae</jats:italic> ) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1→3)-β- <jats:sc>d</jats:sc> -glucans in house dust samples. Metal working fluids spiked with (1→3)-β- <jats:sc>d</jats:sc> -glucans inhibited the glucan assay. Because the assay is specific for (1→6) branched, (1→3)-β- <jats:sc>d</jats:sc> -glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules. </jats:p> |
|---|---|
| Umfang: | 5420-5424 |
| ISSN: |
0099-2240
1098-5336 |
| DOI: | 10.1128/aem.67.12.5420-5424.2001 |