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Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosa...
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Applied and Environmental Microbiology, 65, 1999, 11, S. 4793-4798 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Tran, Huyen L. Fiedler, F. Hodgson, D. A. Kathariou, S. Tran, Huyen L. Fiedler, F. Hodgson, D. A. Kathariou, S. |
|---|---|
| author |
Tran, Huyen L. Fiedler, F. Hodgson, D. A. Kathariou, S. |
| spellingShingle |
Tran, Huyen L. Fiedler, F. Hodgson, D. A. Kathariou, S. Applied and Environmental Microbiology Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
tran, huyen l. |
| spelling |
Tran, Huyen L. Fiedler, F. Hodgson, D. A. Kathariou, S. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.65.11.4793-4798.1999 <jats:title>ABSTRACT</jats:title> <jats:p> Teichoic acid-associated <jats:italic>N</jats:italic> -acetylglucosamine and rhamnose have been shown to serve as phage receptors in <jats:italic>Listeria monocytogenes</jats:italic> serotype 1/2a. We generated and characterized two single-copy Tn <jats:italic>916</jats:italic> ΔE mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified <jats:italic>ptsHI</jats:italic> locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was <jats:italic>clpC</jats:italic> , involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in <jats:italic>N</jats:italic> -acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected. </jats:p> Transposon-Induced Mutations in Two Loci of <i>Listeria monocytogenes</i> Serotype 1/2a Result in Phage Resistance and Lack of <i>N</i> -Acetylglucosamine in the Teichoic Acid of the Cell Wall Applied and Environmental Microbiology |
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American Society for Microbiology, 1999 |
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American Society for Microbiology, 1999 |
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Applied and Environmental Microbiology |
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| title |
Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_unstemmed |
Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_full |
Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_fullStr |
Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_full_unstemmed |
Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_short |
Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_sort |
transposon-induced mutations in two loci of
<i>listeria monocytogenes</i>
serotype 1/2a result in phage resistance and lack of
<i>n</i>
-acetylglucosamine in the teichoic acid of the cell wall |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.65.11.4793-4798.1999 |
| publishDate |
1999 |
| physical |
4793-4798 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Teichoic acid-associated
<jats:italic>N</jats:italic>
-acetylglucosamine and rhamnose have been shown to serve as phage receptors in
<jats:italic>Listeria monocytogenes</jats:italic>
serotype 1/2a. We generated and characterized two single-copy Tn
<jats:italic>916</jats:italic>
ΔE mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified
<jats:italic>ptsHI</jats:italic>
locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was
<jats:italic>clpC</jats:italic>
, involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in
<jats:italic>N</jats:italic>
-acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected.
</jats:p> |
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| author | Tran, Huyen L., Fiedler, F., Hodgson, D. A., Kathariou, S. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Teichoic acid-associated <jats:italic>N</jats:italic> -acetylglucosamine and rhamnose have been shown to serve as phage receptors in <jats:italic>Listeria monocytogenes</jats:italic> serotype 1/2a. We generated and characterized two single-copy Tn <jats:italic>916</jats:italic> ΔE mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified <jats:italic>ptsHI</jats:italic> locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was <jats:italic>clpC</jats:italic> , involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in <jats:italic>N</jats:italic> -acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected. </jats:p> |
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| imprint_str_mv | American Society for Microbiology, 1999 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11 |
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| physical | 4793-4798 |
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| spelling | Tran, Huyen L. Fiedler, F. Hodgson, D. A. Kathariou, S. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.65.11.4793-4798.1999 <jats:title>ABSTRACT</jats:title> <jats:p> Teichoic acid-associated <jats:italic>N</jats:italic> -acetylglucosamine and rhamnose have been shown to serve as phage receptors in <jats:italic>Listeria monocytogenes</jats:italic> serotype 1/2a. We generated and characterized two single-copy Tn <jats:italic>916</jats:italic> ΔE mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified <jats:italic>ptsHI</jats:italic> locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was <jats:italic>clpC</jats:italic> , involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in <jats:italic>N</jats:italic> -acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected. </jats:p> Transposon-Induced Mutations in Two Loci of <i>Listeria monocytogenes</i> Serotype 1/2a Result in Phage Resistance and Lack of <i>N</i> -Acetylglucosamine in the Teichoic Acid of the Cell Wall Applied and Environmental Microbiology |
| spellingShingle | Tran, Huyen L., Fiedler, F., Hodgson, D. A., Kathariou, S., Applied and Environmental Microbiology, Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_full | Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_fullStr | Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_full_unstemmed | Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_short | Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| title_sort | transposon-induced mutations in two loci of <i>listeria monocytogenes</i> serotype 1/2a result in phage resistance and lack of <i>n</i> -acetylglucosamine in the teichoic acid of the cell wall |
| title_unstemmed | Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosamine in the Teichoic Acid of the Cell Wall |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.65.11.4793-4798.1999 |