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Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N -Acetylglucosa...
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
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| Personen und Körperschaften: | , , , |
| In: | Applied and Environmental Microbiology, 65, 1999, 11, S. 4793-4798 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> Teichoic acid-associated <jats:italic>N</jats:italic> -acetylglucosamine and rhamnose have been shown to serve as phage receptors in <jats:italic>Listeria monocytogenes</jats:italic> serotype 1/2a. We generated and characterized two single-copy Tn <jats:italic>916</jats:italic> ΔE mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified <jats:italic>ptsHI</jats:italic> locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was <jats:italic>clpC</jats:italic> , involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in <jats:italic>N</jats:italic> -acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected. </jats:p> |
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| Umfang: | 4793-4798 |
| ISSN: |
0099-2240
1098-5336 |
| DOI: | 10.1128/aem.65.11.4793-4798.1999 |