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Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Applied and Environmental Microbiology, 56, 1990, 10, S. 3052-3056 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Ossanna, Nina Mischke, Sue Ossanna, Nina Mischke, Sue |
|---|---|
| author |
Ossanna, Nina Mischke, Sue |
| spellingShingle |
Ossanna, Nina Mischke, Sue Applied and Environmental Microbiology Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
ossanna, nina |
| spelling |
Ossanna, Nina Mischke, Sue 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.56.10.3052-3056.1990 <jats:p> Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus <jats:italic>Gliocladium virens</jats:italic> and to transform them to benomyl resistance with a <jats:italic>Neurospora crassa</jats:italic> β-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative and asexual structures demonstrated that no structure of <jats:italic>G. virens</jats:italic> is dependably uninucleate and thus preferentially suitable for transformation. </jats:p> Genetic Transformation of the Biocontrol Fungus <i>Gliocladium virens</i> to Benomyl Resistance Applied and Environmental Microbiology |
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American Society for Microbiology, 1990 |
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American Society for Microbiology, 1990 |
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1098-5336 0099-2240 |
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1990 |
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Applied and Environmental Microbiology |
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| title |
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_unstemmed |
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_full |
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_fullStr |
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_full_unstemmed |
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_short |
Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_sort |
genetic transformation of the biocontrol fungus
<i>gliocladium virens</i>
to benomyl resistance |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.56.10.3052-3056.1990 |
| publishDate |
1990 |
| physical |
3052-3056 |
| description |
<jats:p>
Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus
<jats:italic>Gliocladium virens</jats:italic>
and to transform them to benomyl resistance with a
<jats:italic>Neurospora crassa</jats:italic>
β-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative and asexual structures demonstrated that no structure of
<jats:italic>G. virens</jats:italic>
is dependably uninucleate and thus preferentially suitable for transformation.
</jats:p> |
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| author | Ossanna, Nina, Mischke, Sue |
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| author_sort | ossanna, nina |
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| description | <jats:p> Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus <jats:italic>Gliocladium virens</jats:italic> and to transform them to benomyl resistance with a <jats:italic>Neurospora crassa</jats:italic> β-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative and asexual structures demonstrated that no structure of <jats:italic>G. virens</jats:italic> is dependably uninucleate and thus preferentially suitable for transformation. </jats:p> |
| doi_str_mv | 10.1128/aem.56.10.3052-3056.1990 |
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| imprint | American Society for Microbiology, 1990 |
| imprint_str_mv | American Society for Microbiology, 1990 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 3052-3056 |
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| series | Applied and Environmental Microbiology |
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| spelling | Ossanna, Nina Mischke, Sue 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.56.10.3052-3056.1990 <jats:p> Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus <jats:italic>Gliocladium virens</jats:italic> and to transform them to benomyl resistance with a <jats:italic>Neurospora crassa</jats:italic> β-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative and asexual structures demonstrated that no structure of <jats:italic>G. virens</jats:italic> is dependably uninucleate and thus preferentially suitable for transformation. </jats:p> Genetic Transformation of the Biocontrol Fungus <i>Gliocladium virens</i> to Benomyl Resistance Applied and Environmental Microbiology |
| spellingShingle | Ossanna, Nina, Mischke, Sue, Applied and Environmental Microbiology, Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_full | Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_fullStr | Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_full_unstemmed | Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_short | Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| title_sort | genetic transformation of the biocontrol fungus <i>gliocladium virens</i> to benomyl resistance |
| title_unstemmed | Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.56.10.3052-3056.1990 |