Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance

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Bibliographische Detailangaben
Zeitschriftentitel:	Applied and Environmental Microbiology
Personen und Körperschaften:	Ossanna, Nina, Mischke, Sue
In:	Applied and Environmental Microbiology, 56, 1990, 10, S. 3052-3056
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Ecology Applied Microbiology and Biotechnology Food Science Biotechnology

Details
Zusammenfassung:	<jats:p> Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus <jats:italic>Gliocladium virens</jats:italic> and to transform them to benomyl resistance with a <jats:italic>Neurospora crassa</jats:italic> β-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative and asexual structures demonstrated that no structure of <jats:italic>G. virens</jats:italic> is dependably uninucleate and thus preferentially suitable for transformation. </jats:p>
Umfang:	3052-3056
ISSN:	1098-5336 0099-2240
DOI:	10.1128/aem.56.10.3052-3056.1990