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Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faec...
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| Zeitschriftentitel: | Antimicrobial Agents and Chemotherapy |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Antimicrobial Agents and Chemotherapy, 54, 2010, 11, S. 4744-4749 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Jung, Young-Hee Shin, Eun Shim Kim, Okgene Yoo, Jung Sik Lee, Kyeong Min Yoo, Jae Il Chung, Gyung Tae Lee, Yeong Seon Jung, Young-Hee Shin, Eun Shim Kim, Okgene Yoo, Jung Sik Lee, Kyeong Min Yoo, Jae Il Chung, Gyung Tae Lee, Yeong Seon |
|---|---|
| author |
Jung, Young-Hee Shin, Eun Shim Kim, Okgene Yoo, Jung Sik Lee, Kyeong Min Yoo, Jae Il Chung, Gyung Tae Lee, Yeong Seon |
| spellingShingle |
Jung, Young-Hee Shin, Eun Shim Kim, Okgene Yoo, Jung Sik Lee, Kyeong Min Yoo, Jae Il Chung, Gyung Tae Lee, Yeong Seon Antimicrobial Agents and Chemotherapy Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium Infectious Diseases Pharmacology (medical) Pharmacology |
| author_sort |
jung, young-hee |
| spelling |
Jung, Young-Hee Shin, Eun Shim Kim, Okgene Yoo, Jung Sik Lee, Kyeong Min Yoo, Jae Il Chung, Gyung Tae Lee, Yeong Seon 0066-4804 1098-6596 American Society for Microbiology Infectious Diseases Pharmacology (medical) Pharmacology http://dx.doi.org/10.1128/aac.00798-09 <jats:title>ABSTRACT</jats:title> <jats:p> We characterized two new streptogramin A resistance genes from quinupristin-dalfopristin-resistant <jats:italic>Enterococcus faecium</jats:italic> JS79, which was selected from 79 <jats:italic>E. faecium</jats:italic> isolates lacking known genes encoding streptogramin A acetyltransferase. A 5,650-bp fragment of HindIII-digested plasmid DNA from <jats:italic>E. faecium</jats:italic> JS79 was cloned and sequenced. The fragment contained two open reading frames carrying resistance genes related to streptogramin A, namely, genes for an acetyltransferase and an ATP efflux pump. The first open reading frame comprised 648 bp encoding 216 amino acids with a predicted left-handed parallel β-helix domain structure; this new gene was designated <jats:italic>vatG</jats:italic> . The second open reading frame consisted of 1,575 bp encoding 525 amino acids with two predicted ATPase binding cassette transporters comprised of Walker A, Walker B, and LSSG motifs; this gene was designated <jats:italic>vgaD</jats:italic> . <jats:italic>vgaD</jats:italic> is located 65 bp upstream from <jats:italic>vatG</jats:italic> , was detected together with <jats:italic>vatG</jats:italic> in 12 of 179 quinupristin-dalfopristin-resistant <jats:italic>E. faecium</jats:italic> isolates, and was located on the same plasmid. Also, the 5.6-kb HindIII-digested fragment which was observed in JS79 was detected in nine <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates by Southern hybridization. Therefore, it was expected that these two genes were strongly correlated with each other and that they may be composed of a transposon. Importantly, <jats:italic>vgaD</jats:italic> is the first identified ABC transporter conferring resistance to streptogramin A in <jats:italic>E. faecium</jats:italic> . Pulsed-field gel electrophoresis patterns and sequence types of <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates differed for isolates from humans and nonhumans. </jats:p> Characterization of Two Newly Identified Genes, <i>vgaD</i> and <i>vatG</i> , Conferring Resistance to Streptogramin A in <i>Enterococcus faecium</i> Antimicrobial Agents and Chemotherapy |
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American Society for Microbiology, 2010 |
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American Society for Microbiology, 2010 |
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0066-4804 1098-6596 |
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American Society for Microbiology |
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Antimicrobial Agents and Chemotherapy |
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49 |
| title |
Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_unstemmed |
Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_full |
Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_fullStr |
Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_full_unstemmed |
Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_short |
Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_sort |
characterization of two newly identified genes,
<i>vgad</i>
and
<i>vatg</i>
, conferring resistance to streptogramin a in
<i>enterococcus faecium</i> |
| topic |
Infectious Diseases Pharmacology (medical) Pharmacology |
| url |
http://dx.doi.org/10.1128/aac.00798-09 |
| publishDate |
2010 |
| physical |
4744-4749 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
We characterized two new streptogramin A resistance genes from quinupristin-dalfopristin-resistant
<jats:italic>Enterococcus faecium</jats:italic>
JS79, which was selected from 79
<jats:italic>E. faecium</jats:italic>
isolates lacking known genes encoding streptogramin A acetyltransferase. A 5,650-bp fragment of HindIII-digested plasmid DNA from
<jats:italic>E. faecium</jats:italic>
JS79 was cloned and sequenced. The fragment contained two open reading frames carrying resistance genes related to streptogramin A, namely, genes for an acetyltransferase and an ATP efflux pump. The first open reading frame comprised 648 bp encoding 216 amino acids with a predicted left-handed parallel β-helix domain structure; this new gene was designated
<jats:italic>vatG</jats:italic>
. The second open reading frame consisted of 1,575 bp encoding 525 amino acids with two predicted ATPase binding cassette transporters comprised of Walker A, Walker B, and LSSG motifs; this gene was designated
<jats:italic>vgaD</jats:italic>
.
<jats:italic>vgaD</jats:italic>
is located 65 bp upstream from
<jats:italic>vatG</jats:italic>
, was detected together with
<jats:italic>vatG</jats:italic>
in 12 of 179 quinupristin-dalfopristin-resistant
<jats:italic>E. faecium</jats:italic>
isolates, and was located on the same plasmid. Also, the 5.6-kb HindIII-digested fragment which was observed in JS79 was detected in nine
<jats:italic>vgaD</jats:italic>
- and
<jats:italic>vatG</jats:italic>
-containing
<jats:italic>E. faecium</jats:italic>
isolates by Southern hybridization. Therefore, it was expected that these two genes were strongly correlated with each other and that they may be composed of a transposon. Importantly,
<jats:italic>vgaD</jats:italic>
is the first identified ABC transporter conferring resistance to streptogramin A in
<jats:italic>E. faecium</jats:italic>
. Pulsed-field gel electrophoresis patterns and sequence types of
<jats:italic>vgaD</jats:italic>
- and
<jats:italic>vatG</jats:italic>
-containing
<jats:italic>E. faecium</jats:italic>
isolates differed for isolates from humans and nonhumans.
</jats:p> |
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| author | Jung, Young-Hee, Shin, Eun Shim, Kim, Okgene, Yoo, Jung Sik, Lee, Kyeong Min, Yoo, Jae Il, Chung, Gyung Tae, Lee, Yeong Seon |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> We characterized two new streptogramin A resistance genes from quinupristin-dalfopristin-resistant <jats:italic>Enterococcus faecium</jats:italic> JS79, which was selected from 79 <jats:italic>E. faecium</jats:italic> isolates lacking known genes encoding streptogramin A acetyltransferase. A 5,650-bp fragment of HindIII-digested plasmid DNA from <jats:italic>E. faecium</jats:italic> JS79 was cloned and sequenced. The fragment contained two open reading frames carrying resistance genes related to streptogramin A, namely, genes for an acetyltransferase and an ATP efflux pump. The first open reading frame comprised 648 bp encoding 216 amino acids with a predicted left-handed parallel β-helix domain structure; this new gene was designated <jats:italic>vatG</jats:italic> . The second open reading frame consisted of 1,575 bp encoding 525 amino acids with two predicted ATPase binding cassette transporters comprised of Walker A, Walker B, and LSSG motifs; this gene was designated <jats:italic>vgaD</jats:italic> . <jats:italic>vgaD</jats:italic> is located 65 bp upstream from <jats:italic>vatG</jats:italic> , was detected together with <jats:italic>vatG</jats:italic> in 12 of 179 quinupristin-dalfopristin-resistant <jats:italic>E. faecium</jats:italic> isolates, and was located on the same plasmid. Also, the 5.6-kb HindIII-digested fragment which was observed in JS79 was detected in nine <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates by Southern hybridization. Therefore, it was expected that these two genes were strongly correlated with each other and that they may be composed of a transposon. Importantly, <jats:italic>vgaD</jats:italic> is the first identified ABC transporter conferring resistance to streptogramin A in <jats:italic>E. faecium</jats:italic> . Pulsed-field gel electrophoresis patterns and sequence types of <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates differed for isolates from humans and nonhumans. </jats:p> |
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| spelling | Jung, Young-Hee Shin, Eun Shim Kim, Okgene Yoo, Jung Sik Lee, Kyeong Min Yoo, Jae Il Chung, Gyung Tae Lee, Yeong Seon 0066-4804 1098-6596 American Society for Microbiology Infectious Diseases Pharmacology (medical) Pharmacology http://dx.doi.org/10.1128/aac.00798-09 <jats:title>ABSTRACT</jats:title> <jats:p> We characterized two new streptogramin A resistance genes from quinupristin-dalfopristin-resistant <jats:italic>Enterococcus faecium</jats:italic> JS79, which was selected from 79 <jats:italic>E. faecium</jats:italic> isolates lacking known genes encoding streptogramin A acetyltransferase. A 5,650-bp fragment of HindIII-digested plasmid DNA from <jats:italic>E. faecium</jats:italic> JS79 was cloned and sequenced. The fragment contained two open reading frames carrying resistance genes related to streptogramin A, namely, genes for an acetyltransferase and an ATP efflux pump. The first open reading frame comprised 648 bp encoding 216 amino acids with a predicted left-handed parallel β-helix domain structure; this new gene was designated <jats:italic>vatG</jats:italic> . The second open reading frame consisted of 1,575 bp encoding 525 amino acids with two predicted ATPase binding cassette transporters comprised of Walker A, Walker B, and LSSG motifs; this gene was designated <jats:italic>vgaD</jats:italic> . <jats:italic>vgaD</jats:italic> is located 65 bp upstream from <jats:italic>vatG</jats:italic> , was detected together with <jats:italic>vatG</jats:italic> in 12 of 179 quinupristin-dalfopristin-resistant <jats:italic>E. faecium</jats:italic> isolates, and was located on the same plasmid. Also, the 5.6-kb HindIII-digested fragment which was observed in JS79 was detected in nine <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates by Southern hybridization. Therefore, it was expected that these two genes were strongly correlated with each other and that they may be composed of a transposon. Importantly, <jats:italic>vgaD</jats:italic> is the first identified ABC transporter conferring resistance to streptogramin A in <jats:italic>E. faecium</jats:italic> . Pulsed-field gel electrophoresis patterns and sequence types of <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates differed for isolates from humans and nonhumans. </jats:p> Characterization of Two Newly Identified Genes, <i>vgaD</i> and <i>vatG</i> , Conferring Resistance to Streptogramin A in <i>Enterococcus faecium</i> Antimicrobial Agents and Chemotherapy |
| spellingShingle | Jung, Young-Hee, Shin, Eun Shim, Kim, Okgene, Yoo, Jung Sik, Lee, Kyeong Min, Yoo, Jae Il, Chung, Gyung Tae, Lee, Yeong Seon, Antimicrobial Agents and Chemotherapy, Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium, Infectious Diseases, Pharmacology (medical), Pharmacology |
| title | Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_full | Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_fullStr | Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_full_unstemmed | Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_short | Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| title_sort | characterization of two newly identified genes, <i>vgad</i> and <i>vatg</i> , conferring resistance to streptogramin a in <i>enterococcus faecium</i> |
| title_unstemmed | Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faecium |
| topic | Infectious Diseases, Pharmacology (medical), Pharmacology |
| url | http://dx.doi.org/10.1128/aac.00798-09 |