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Characterization of Two Newly Identified Genes, vgaD and vatG , Conferring Resistance to Streptogramin A in Enterococcus faec...
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| Zeitschriftentitel: | Antimicrobial Agents and Chemotherapy |
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| Personen und Körperschaften: | , , , , , , , |
| In: | Antimicrobial Agents and Chemotherapy, 54, 2010, 11, S. 4744-4749 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> We characterized two new streptogramin A resistance genes from quinupristin-dalfopristin-resistant <jats:italic>Enterococcus faecium</jats:italic> JS79, which was selected from 79 <jats:italic>E. faecium</jats:italic> isolates lacking known genes encoding streptogramin A acetyltransferase. A 5,650-bp fragment of HindIII-digested plasmid DNA from <jats:italic>E. faecium</jats:italic> JS79 was cloned and sequenced. The fragment contained two open reading frames carrying resistance genes related to streptogramin A, namely, genes for an acetyltransferase and an ATP efflux pump. The first open reading frame comprised 648 bp encoding 216 amino acids with a predicted left-handed parallel β-helix domain structure; this new gene was designated <jats:italic>vatG</jats:italic> . The second open reading frame consisted of 1,575 bp encoding 525 amino acids with two predicted ATPase binding cassette transporters comprised of Walker A, Walker B, and LSSG motifs; this gene was designated <jats:italic>vgaD</jats:italic> . <jats:italic>vgaD</jats:italic> is located 65 bp upstream from <jats:italic>vatG</jats:italic> , was detected together with <jats:italic>vatG</jats:italic> in 12 of 179 quinupristin-dalfopristin-resistant <jats:italic>E. faecium</jats:italic> isolates, and was located on the same plasmid. Also, the 5.6-kb HindIII-digested fragment which was observed in JS79 was detected in nine <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates by Southern hybridization. Therefore, it was expected that these two genes were strongly correlated with each other and that they may be composed of a transposon. Importantly, <jats:italic>vgaD</jats:italic> is the first identified ABC transporter conferring resistance to streptogramin A in <jats:italic>E. faecium</jats:italic> . Pulsed-field gel electrophoresis patterns and sequence types of <jats:italic>vgaD</jats:italic> - and <jats:italic>vatG</jats:italic> -containing <jats:italic>E. faecium</jats:italic> isolates differed for isolates from humans and nonhumans. </jats:p> |
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| Umfang: | 4744-4749 |
| ISSN: |
0066-4804
1098-6596 |
| DOI: | 10.1128/aac.00798-09 |