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Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece
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| Zeitschriftentitel: | Plant Pathology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Plant Pathology, 64, 2015, 4, S. 792-798 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Varveri, C. Olmos, A. Pina, J. A. Marroquín, C. Cambra, M. Varveri, C. Olmos, A. Pina, J. A. Marroquín, C. Cambra, M. |
|---|---|
| author |
Varveri, C. Olmos, A. Pina, J. A. Marroquín, C. Cambra, M. |
| spellingShingle |
Varveri, C. Olmos, A. Pina, J. A. Marroquín, C. Cambra, M. Plant Pathology Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece Horticulture Plant Science Genetics Agronomy and Crop Science |
| author_sort |
varveri, c. |
| spelling |
Varveri, C. Olmos, A. Pina, J. A. Marroquín, C. Cambra, M. 0032-0862 1365-3059 Wiley Horticulture Plant Science Genetics Agronomy and Crop Science http://dx.doi.org/10.1111/ppa.12308 <jats:p>A <jats:italic>Citrus tristeza virus</jats:italic> (<jats:styled-content style="fixed-case">CTV</jats:styled-content>) isolate (L192<jats:styled-content style="fixed-case">GR</jats:styled-content>) naturally occurring in lemon trees of more than 100 years old in Greece was fully characterized. Virus‐derived small interfering <jats:styled-content style="fixed-case">RNA</jats:styled-content>s, induced by Dicer processing of ds<jats:styled-content style="fixed-case">RNA</jats:styled-content>s formed during <jats:styled-content style="fixed-case">RNA</jats:styled-content> virus replication, were isolated and used as targets for sequencing. Next‐generation high‐throughput sequencing using the Ion Torrent platform was performed. A total of 432 632 sequences, 94·05% of which corresponded to L192<jats:styled-content style="fixed-case">GR</jats:styled-content>, were determined. Subsequent bioinformatics analysis enabled the determination of the full‐length 19 251 nt genome of the L192<jats:styled-content style="fixed-case">GR</jats:styled-content> isolate (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="KC262793">KC262793</jats:ext-link>). Comparative analysis of complete genomes revealed molecular homology with <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> isolate <jats:styled-content style="fixed-case">FS</jats:styled-content>2‐2 from Florida (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="EU937519">EU937519</jats:ext-link>) with 98·2% nucleotide sequence identity. Recombination events were detected in L192GR and they probably contribute to its unique characteristics. Specifically, although most isolates of the <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> group induce the seedling yellows syndrome and react positively with the monoclonal antibody <jats:styled-content style="fixed-case">MCA</jats:styled-content>13, which is typically associated with severe <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolates, the <jats:styled-content style="fixed-case">MCA</jats:styled-content>13‐positive L192<jats:styled-content style="fixed-case">GR</jats:styled-content> gave very mild or even no symptoms in the seedling yellows indicator plants. Furthermore, experimental aphid transmissibility studies revealed a poor transmission efficiency of 20%. This is the first report of a <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolate originating from a lemon tree being fully characterized at biological, serological and molecular levels. The present study further confirms that, when the goal is the risk assessment associated with a new pathogen or isolate in a particular area, molecular data have to be combined with the biological properties of the pathogen.</jats:p> Biological and molecular characterization of a distinct <i>Citrus tristeza virus</i> isolate originating from a lemon tree in Greece Plant Pathology |
| doi_str_mv |
10.1111/ppa.12308 |
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Online Free |
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Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft Biologie |
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| imprint |
Wiley, 2015 |
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Wiley, 2015 |
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0032-0862 1365-3059 |
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2015 |
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Plant Pathology |
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49 |
| title |
Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_unstemmed |
Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_full |
Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_fullStr |
Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_full_unstemmed |
Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_short |
Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_sort |
biological and molecular characterization of a distinct <i>citrus tristeza virus</i> isolate originating from a lemon tree in greece |
| topic |
Horticulture Plant Science Genetics Agronomy and Crop Science |
| url |
http://dx.doi.org/10.1111/ppa.12308 |
| publishDate |
2015 |
| physical |
792-798 |
| description |
<jats:p>A <jats:italic>Citrus tristeza virus</jats:italic> (<jats:styled-content style="fixed-case">CTV</jats:styled-content>) isolate (L192<jats:styled-content style="fixed-case">GR</jats:styled-content>) naturally occurring in lemon trees of more than 100 years old in Greece was fully characterized. Virus‐derived small interfering <jats:styled-content style="fixed-case">RNA</jats:styled-content>s, induced by Dicer processing of ds<jats:styled-content style="fixed-case">RNA</jats:styled-content>s formed during <jats:styled-content style="fixed-case">RNA</jats:styled-content> virus replication, were isolated and used as targets for sequencing. Next‐generation high‐throughput sequencing using the Ion Torrent platform was performed. A total of 432 632 sequences, 94·05% of which corresponded to L192<jats:styled-content style="fixed-case">GR</jats:styled-content>, were determined. Subsequent bioinformatics analysis enabled the determination of the full‐length 19 251 nt genome of the L192<jats:styled-content style="fixed-case">GR</jats:styled-content> isolate (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="KC262793">KC262793</jats:ext-link>). Comparative analysis of complete genomes revealed molecular homology with <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> isolate <jats:styled-content style="fixed-case">FS</jats:styled-content>2‐2 from Florida (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="EU937519">EU937519</jats:ext-link>) with 98·2% nucleotide sequence identity. Recombination events were detected in L192GR and they probably contribute to its unique characteristics. Specifically, although most isolates of the <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> group induce the seedling yellows syndrome and react positively with the monoclonal antibody <jats:styled-content style="fixed-case">MCA</jats:styled-content>13, which is typically associated with severe <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolates, the <jats:styled-content style="fixed-case">MCA</jats:styled-content>13‐positive L192<jats:styled-content style="fixed-case">GR</jats:styled-content> gave very mild or even no symptoms in the seedling yellows indicator plants. Furthermore, experimental aphid transmissibility studies revealed a poor transmission efficiency of 20%. This is the first report of a <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolate originating from a lemon tree being fully characterized at biological, serological and molecular levels. The present study further confirms that, when the goal is the risk assessment associated with a new pathogen or isolate in a particular area, molecular data have to be combined with the biological properties of the pathogen.</jats:p> |
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| author | Varveri, C., Olmos, A., Pina, J. A., Marroquín, C., Cambra, M. |
| author_facet | Varveri, C., Olmos, A., Pina, J. A., Marroquín, C., Cambra, M., Varveri, C., Olmos, A., Pina, J. A., Marroquín, C., Cambra, M. |
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| description | <jats:p>A <jats:italic>Citrus tristeza virus</jats:italic> (<jats:styled-content style="fixed-case">CTV</jats:styled-content>) isolate (L192<jats:styled-content style="fixed-case">GR</jats:styled-content>) naturally occurring in lemon trees of more than 100 years old in Greece was fully characterized. Virus‐derived small interfering <jats:styled-content style="fixed-case">RNA</jats:styled-content>s, induced by Dicer processing of ds<jats:styled-content style="fixed-case">RNA</jats:styled-content>s formed during <jats:styled-content style="fixed-case">RNA</jats:styled-content> virus replication, were isolated and used as targets for sequencing. Next‐generation high‐throughput sequencing using the Ion Torrent platform was performed. A total of 432 632 sequences, 94·05% of which corresponded to L192<jats:styled-content style="fixed-case">GR</jats:styled-content>, were determined. Subsequent bioinformatics analysis enabled the determination of the full‐length 19 251 nt genome of the L192<jats:styled-content style="fixed-case">GR</jats:styled-content> isolate (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="KC262793">KC262793</jats:ext-link>). Comparative analysis of complete genomes revealed molecular homology with <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> isolate <jats:styled-content style="fixed-case">FS</jats:styled-content>2‐2 from Florida (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="EU937519">EU937519</jats:ext-link>) with 98·2% nucleotide sequence identity. Recombination events were detected in L192GR and they probably contribute to its unique characteristics. Specifically, although most isolates of the <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> group induce the seedling yellows syndrome and react positively with the monoclonal antibody <jats:styled-content style="fixed-case">MCA</jats:styled-content>13, which is typically associated with severe <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolates, the <jats:styled-content style="fixed-case">MCA</jats:styled-content>13‐positive L192<jats:styled-content style="fixed-case">GR</jats:styled-content> gave very mild or even no symptoms in the seedling yellows indicator plants. Furthermore, experimental aphid transmissibility studies revealed a poor transmission efficiency of 20%. This is the first report of a <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolate originating from a lemon tree being fully characterized at biological, serological and molecular levels. The present study further confirms that, when the goal is the risk assessment associated with a new pathogen or isolate in a particular area, molecular data have to be combined with the biological properties of the pathogen.</jats:p> |
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| spelling | Varveri, C. Olmos, A. Pina, J. A. Marroquín, C. Cambra, M. 0032-0862 1365-3059 Wiley Horticulture Plant Science Genetics Agronomy and Crop Science http://dx.doi.org/10.1111/ppa.12308 <jats:p>A <jats:italic>Citrus tristeza virus</jats:italic> (<jats:styled-content style="fixed-case">CTV</jats:styled-content>) isolate (L192<jats:styled-content style="fixed-case">GR</jats:styled-content>) naturally occurring in lemon trees of more than 100 years old in Greece was fully characterized. Virus‐derived small interfering <jats:styled-content style="fixed-case">RNA</jats:styled-content>s, induced by Dicer processing of ds<jats:styled-content style="fixed-case">RNA</jats:styled-content>s formed during <jats:styled-content style="fixed-case">RNA</jats:styled-content> virus replication, were isolated and used as targets for sequencing. Next‐generation high‐throughput sequencing using the Ion Torrent platform was performed. A total of 432 632 sequences, 94·05% of which corresponded to L192<jats:styled-content style="fixed-case">GR</jats:styled-content>, were determined. Subsequent bioinformatics analysis enabled the determination of the full‐length 19 251 nt genome of the L192<jats:styled-content style="fixed-case">GR</jats:styled-content> isolate (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="KC262793">KC262793</jats:ext-link>). Comparative analysis of complete genomes revealed molecular homology with <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> isolate <jats:styled-content style="fixed-case">FS</jats:styled-content>2‐2 from Florida (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="EU937519">EU937519</jats:ext-link>) with 98·2% nucleotide sequence identity. Recombination events were detected in L192GR and they probably contribute to its unique characteristics. Specifically, although most isolates of the <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> group induce the seedling yellows syndrome and react positively with the monoclonal antibody <jats:styled-content style="fixed-case">MCA</jats:styled-content>13, which is typically associated with severe <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolates, the <jats:styled-content style="fixed-case">MCA</jats:styled-content>13‐positive L192<jats:styled-content style="fixed-case">GR</jats:styled-content> gave very mild or even no symptoms in the seedling yellows indicator plants. Furthermore, experimental aphid transmissibility studies revealed a poor transmission efficiency of 20%. This is the first report of a <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolate originating from a lemon tree being fully characterized at biological, serological and molecular levels. The present study further confirms that, when the goal is the risk assessment associated with a new pathogen or isolate in a particular area, molecular data have to be combined with the biological properties of the pathogen.</jats:p> Biological and molecular characterization of a distinct <i>Citrus tristeza virus</i> isolate originating from a lemon tree in Greece Plant Pathology |
| spellingShingle | Varveri, C., Olmos, A., Pina, J. A., Marroquín, C., Cambra, M., Plant Pathology, Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece, Horticulture, Plant Science, Genetics, Agronomy and Crop Science |
| title | Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_full | Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_fullStr | Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_full_unstemmed | Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_short | Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| title_sort | biological and molecular characterization of a distinct <i>citrus tristeza virus</i> isolate originating from a lemon tree in greece |
| title_unstemmed | Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece |
| topic | Horticulture, Plant Science, Genetics, Agronomy and Crop Science |
| url | http://dx.doi.org/10.1111/ppa.12308 |