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Biological and molecular characterization of a distinct Citrus tristeza virus isolate originating from a lemon tree in Greece
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| Zeitschriftentitel: | Plant Pathology |
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| Personen und Körperschaften: | , , , , |
| In: | Plant Pathology, 64, 2015, 4, S. 792-798 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| Zusammenfassung: | <jats:p>A <jats:italic>Citrus tristeza virus</jats:italic> (<jats:styled-content style="fixed-case">CTV</jats:styled-content>) isolate (L192<jats:styled-content style="fixed-case">GR</jats:styled-content>) naturally occurring in lemon trees of more than 100 years old in Greece was fully characterized. Virus‐derived small interfering <jats:styled-content style="fixed-case">RNA</jats:styled-content>s, induced by Dicer processing of ds<jats:styled-content style="fixed-case">RNA</jats:styled-content>s formed during <jats:styled-content style="fixed-case">RNA</jats:styled-content> virus replication, were isolated and used as targets for sequencing. Next‐generation high‐throughput sequencing using the Ion Torrent platform was performed. A total of 432 632 sequences, 94·05% of which corresponded to L192<jats:styled-content style="fixed-case">GR</jats:styled-content>, were determined. Subsequent bioinformatics analysis enabled the determination of the full‐length 19 251 nt genome of the L192<jats:styled-content style="fixed-case">GR</jats:styled-content> isolate (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="KC262793">KC262793</jats:ext-link>). Comparative analysis of complete genomes revealed molecular homology with <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> isolate <jats:styled-content style="fixed-case">FS</jats:styled-content>2‐2 from Florida (GenBank no. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="DDBJ/EMBL/GenBank" xlink:href="EU937519">EU937519</jats:ext-link>) with 98·2% nucleotide sequence identity. Recombination events were detected in L192GR and they probably contribute to its unique characteristics. Specifically, although most isolates of the <jats:styled-content style="fixed-case">CTV</jats:styled-content>‐<jats:styled-content style="fixed-case">VT</jats:styled-content> group induce the seedling yellows syndrome and react positively with the monoclonal antibody <jats:styled-content style="fixed-case">MCA</jats:styled-content>13, which is typically associated with severe <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolates, the <jats:styled-content style="fixed-case">MCA</jats:styled-content>13‐positive L192<jats:styled-content style="fixed-case">GR</jats:styled-content> gave very mild or even no symptoms in the seedling yellows indicator plants. Furthermore, experimental aphid transmissibility studies revealed a poor transmission efficiency of 20%. This is the first report of a <jats:styled-content style="fixed-case">CTV</jats:styled-content> isolate originating from a lemon tree being fully characterized at biological, serological and molecular levels. The present study further confirms that, when the goal is the risk assessment associated with a new pathogen or isolate in a particular area, molecular data have to be combined with the biological properties of the pathogen.</jats:p> |
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| Umfang: | 792-798 |
| ISSN: |
0032-0862
1365-3059 |
| DOI: | 10.1111/ppa.12308 |