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Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate
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Zeitschriftentitel: | The FEBS Journal |
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Personen und Körperschaften: | , , , |
In: | The FEBS Journal, 272, 2005, 10, S. 2416-2427 |
Format: | E-Article |
Sprache: | Englisch |
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Wiley
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author_facet |
Nouaille, Régis Matulova, Maria Delort, Anne‐Marie Forano, Evelyne Nouaille, Régis Matulova, Maria Delort, Anne‐Marie Forano, Evelyne |
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author |
Nouaille, Régis Matulova, Maria Delort, Anne‐Marie Forano, Evelyne |
spellingShingle |
Nouaille, Régis Matulova, Maria Delort, Anne‐Marie Forano, Evelyne The FEBS Journal Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate Cell Biology Molecular Biology Biochemistry |
author_sort |
nouaille, régis |
spelling |
Nouaille, Régis Matulova, Maria Delort, Anne‐Marie Forano, Evelyne 1742-464X 1742-4658 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1111/j.1742-4658.2005.04662.x <jats:p>In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in<jats:italic>Fibrobacter succinogenes</jats:italic>S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by<jats:sup>1</jats:sup>H‐NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin‐1‐phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.</jats:p> Oligosaccharide synthesis in<i>Fibrobacter succinogenes</i>S85 and its modulation by the substrate The FEBS Journal |
doi_str_mv |
10.1111/j.1742-4658.2005.04662.x |
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Biologie Chemie und Pharmazie |
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Wiley, 2005 |
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Wiley |
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The FEBS Journal |
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title |
Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_unstemmed |
Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_full |
Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_fullStr |
Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_full_unstemmed |
Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_short |
Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_sort |
oligosaccharide synthesis in<i>fibrobacter succinogenes</i>s85 and its modulation by the substrate |
topic |
Cell Biology Molecular Biology Biochemistry |
url |
http://dx.doi.org/10.1111/j.1742-4658.2005.04662.x |
publishDate |
2005 |
physical |
2416-2427 |
description |
<jats:p>In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in<jats:italic>Fibrobacter succinogenes</jats:italic>S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by<jats:sup>1</jats:sup>H‐NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin‐1‐phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.</jats:p> |
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author | Nouaille, Régis, Matulova, Maria, Delort, Anne‐Marie, Forano, Evelyne |
author_facet | Nouaille, Régis, Matulova, Maria, Delort, Anne‐Marie, Forano, Evelyne, Nouaille, Régis, Matulova, Maria, Delort, Anne‐Marie, Forano, Evelyne |
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container_title | The FEBS Journal |
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description | <jats:p>In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in<jats:italic>Fibrobacter succinogenes</jats:italic>S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by<jats:sup>1</jats:sup>H‐NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin‐1‐phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.</jats:p> |
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spelling | Nouaille, Régis Matulova, Maria Delort, Anne‐Marie Forano, Evelyne 1742-464X 1742-4658 Wiley Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1111/j.1742-4658.2005.04662.x <jats:p>In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in<jats:italic>Fibrobacter succinogenes</jats:italic>S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by<jats:sup>1</jats:sup>H‐NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin‐1‐phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.</jats:p> Oligosaccharide synthesis in<i>Fibrobacter succinogenes</i>S85 and its modulation by the substrate The FEBS Journal |
spellingShingle | Nouaille, Régis, Matulova, Maria, Delort, Anne‐Marie, Forano, Evelyne, The FEBS Journal, Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate, Cell Biology, Molecular Biology, Biochemistry |
title | Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_full | Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_fullStr | Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_full_unstemmed | Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_short | Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
title_sort | oligosaccharide synthesis in<i>fibrobacter succinogenes</i>s85 and its modulation by the substrate |
title_unstemmed | Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate |
topic | Cell Biology, Molecular Biology, Biochemistry |
url | http://dx.doi.org/10.1111/j.1742-4658.2005.04662.x |