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Oligosaccharide synthesis inFibrobacter succinogenesS85 and its modulation by the substrate
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| Zeitschriftentitel: | The FEBS Journal |
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| Personen und Körperschaften: | , , , |
| In: | The FEBS Journal, 272, 2005, 10, S. 2416-2427 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| Zusammenfassung: | <jats:p>In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in<jats:italic>Fibrobacter succinogenes</jats:italic>S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by<jats:sup>1</jats:sup>H‐NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin‐1‐phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.</jats:p> |
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| Umfang: | 2416-2427 |
| ISSN: |
1742-464X
1742-4658 |
| DOI: | 10.1111/j.1742-4658.2005.04662.x |