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Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin
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| Zeitschriftentitel: | Cell Proliferation |
|---|---|
| Personen und Körperschaften: | , |
| In: | Cell Proliferation, 47, 2014, 2, S. 124-132 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Zhou, C. Lin, Y. Zhou, C. Lin, Y. |
|---|---|
| author |
Zhou, C. Lin, Y. |
| spellingShingle |
Zhou, C. Lin, Y. Cell Proliferation Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin Cell Biology General Medicine |
| author_sort |
zhou, c. |
| spelling |
Zhou, C. Lin, Y. 0960-7722 1365-2184 Wiley Cell Biology General Medicine http://dx.doi.org/10.1111/cpr.12097 <jats:title>Abstract</jats:title><jats:sec><jats:title>Objectives</jats:title><jats:p>The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (<jats:styled-content style="fixed-case">mASC</jats:styled-content>s) <jats:italic>in vitro</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Materials and methods</jats:title><jats:p>Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercetin on cell proliferation were assessed using <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay. Then they were treated by quercetin for 3, 7 and 11 days in a range of concentrations. Finally, effects of quercetin on osteogenic differentiation of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s were analysed by real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Data of <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay showed that quercetin did not enhance <jats:styled-content style="fixed-case">mASC</jats:styled-content> proliferation in a dose‐dependent or time‐dependent manner. Results of <jats:styled-content style="fixed-case">qPCR</jats:styled-content> indicated that quercetin promoted expressions of Osx, Runx2, BMP‐2, Col‐1, OPN and OCN at the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> level in the presence of osteo‐induction medium.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Our data demonstrated that quercetin was not active in terms of enhancing <jats:styled-content style="fixed-case">mASC</jats:styled-content>s proliferation; however, it increased osteogenesis of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s by up‐regulation of genes including <jats:italic>Osx</jats:italic>,<jats:italic> Runx2</jats:italic>,<jats:italic> BMP‐2</jats:italic>,<jats:italic> Col‐1</jats:italic>,<jats:italic> OPN</jats:italic> and <jats:italic>OCN</jats:italic>.</jats:p></jats:sec> Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin Cell Proliferation |
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10.1111/cpr.12097 |
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Wiley, 2014 |
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Wiley, 2014 |
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2014 |
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Cell Proliferation |
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| title |
Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_unstemmed |
Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_full |
Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_fullStr |
Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_full_unstemmed |
Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_short |
Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_sort |
osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| topic |
Cell Biology General Medicine |
| url |
http://dx.doi.org/10.1111/cpr.12097 |
| publishDate |
2014 |
| physical |
124-132 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:title>Objectives</jats:title><jats:p>The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (<jats:styled-content style="fixed-case">mASC</jats:styled-content>s) <jats:italic>in vitro</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Materials and methods</jats:title><jats:p>Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercetin on cell proliferation were assessed using <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay. Then they were treated by quercetin for 3, 7 and 11 days in a range of concentrations. Finally, effects of quercetin on osteogenic differentiation of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s were analysed by real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Data of <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay showed that quercetin did not enhance <jats:styled-content style="fixed-case">mASC</jats:styled-content> proliferation in a dose‐dependent or time‐dependent manner. Results of <jats:styled-content style="fixed-case">qPCR</jats:styled-content> indicated that quercetin promoted expressions of Osx, Runx2, BMP‐2, Col‐1, OPN and OCN at the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> level in the presence of osteo‐induction medium.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Our data demonstrated that quercetin was not active in terms of enhancing <jats:styled-content style="fixed-case">mASC</jats:styled-content>s proliferation; however, it increased osteogenesis of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s by up‐regulation of genes including <jats:italic>Osx</jats:italic>,<jats:italic> Runx2</jats:italic>,<jats:italic> BMP‐2</jats:italic>,<jats:italic> Col‐1</jats:italic>,<jats:italic> OPN</jats:italic> and <jats:italic>OCN</jats:italic>.</jats:p></jats:sec> |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:title>Objectives</jats:title><jats:p>The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (<jats:styled-content style="fixed-case">mASC</jats:styled-content>s) <jats:italic>in vitro</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Materials and methods</jats:title><jats:p>Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercetin on cell proliferation were assessed using <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay. Then they were treated by quercetin for 3, 7 and 11 days in a range of concentrations. Finally, effects of quercetin on osteogenic differentiation of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s were analysed by real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Data of <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay showed that quercetin did not enhance <jats:styled-content style="fixed-case">mASC</jats:styled-content> proliferation in a dose‐dependent or time‐dependent manner. Results of <jats:styled-content style="fixed-case">qPCR</jats:styled-content> indicated that quercetin promoted expressions of Osx, Runx2, BMP‐2, Col‐1, OPN and OCN at the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> level in the presence of osteo‐induction medium.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Our data demonstrated that quercetin was not active in terms of enhancing <jats:styled-content style="fixed-case">mASC</jats:styled-content>s proliferation; however, it increased osteogenesis of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s by up‐regulation of genes including <jats:italic>Osx</jats:italic>,<jats:italic> Runx2</jats:italic>,<jats:italic> BMP‐2</jats:italic>,<jats:italic> Col‐1</jats:italic>,<jats:italic> OPN</jats:italic> and <jats:italic>OCN</jats:italic>.</jats:p></jats:sec> |
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| imprint | Wiley, 2014 |
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| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Zhou, C. Lin, Y. 0960-7722 1365-2184 Wiley Cell Biology General Medicine http://dx.doi.org/10.1111/cpr.12097 <jats:title>Abstract</jats:title><jats:sec><jats:title>Objectives</jats:title><jats:p>The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (<jats:styled-content style="fixed-case">mASC</jats:styled-content>s) <jats:italic>in vitro</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Materials and methods</jats:title><jats:p>Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercetin on cell proliferation were assessed using <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay. Then they were treated by quercetin for 3, 7 and 11 days in a range of concentrations. Finally, effects of quercetin on osteogenic differentiation of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s were analysed by real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Data of <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay showed that quercetin did not enhance <jats:styled-content style="fixed-case">mASC</jats:styled-content> proliferation in a dose‐dependent or time‐dependent manner. Results of <jats:styled-content style="fixed-case">qPCR</jats:styled-content> indicated that quercetin promoted expressions of Osx, Runx2, BMP‐2, Col‐1, OPN and OCN at the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> level in the presence of osteo‐induction medium.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Our data demonstrated that quercetin was not active in terms of enhancing <jats:styled-content style="fixed-case">mASC</jats:styled-content>s proliferation; however, it increased osteogenesis of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s by up‐regulation of genes including <jats:italic>Osx</jats:italic>,<jats:italic> Runx2</jats:italic>,<jats:italic> BMP‐2</jats:italic>,<jats:italic> Col‐1</jats:italic>,<jats:italic> OPN</jats:italic> and <jats:italic>OCN</jats:italic>.</jats:p></jats:sec> Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin Cell Proliferation |
| spellingShingle | Zhou, C., Lin, Y., Cell Proliferation, Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin, Cell Biology, General Medicine |
| title | Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_full | Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_fullStr | Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_full_unstemmed | Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_short | Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_sort | osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| title_unstemmed | Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin |
| topic | Cell Biology, General Medicine |
| url | http://dx.doi.org/10.1111/cpr.12097 |