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Osteogenic differentiation of adipose‐derived stem cells promoted by quercetin
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| Zeitschriftentitel: | Cell Proliferation |
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| Personen und Körperschaften: | , |
| In: | Cell Proliferation, 47, 2014, 2, S. 124-132 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>Abstract</jats:title><jats:sec><jats:title>Objectives</jats:title><jats:p>The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (<jats:styled-content style="fixed-case">mASC</jats:styled-content>s) <jats:italic>in vitro</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Materials and methods</jats:title><jats:p>Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercetin on cell proliferation were assessed using <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay. Then they were treated by quercetin for 3, 7 and 11 days in a range of concentrations. Finally, effects of quercetin on osteogenic differentiation of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s were analysed by real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Data of <jats:styled-content style="fixed-case">MTT</jats:styled-content> assay showed that quercetin did not enhance <jats:styled-content style="fixed-case">mASC</jats:styled-content> proliferation in a dose‐dependent or time‐dependent manner. Results of <jats:styled-content style="fixed-case">qPCR</jats:styled-content> indicated that quercetin promoted expressions of Osx, Runx2, BMP‐2, Col‐1, OPN and OCN at the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> level in the presence of osteo‐induction medium.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Our data demonstrated that quercetin was not active in terms of enhancing <jats:styled-content style="fixed-case">mASC</jats:styled-content>s proliferation; however, it increased osteogenesis of <jats:styled-content style="fixed-case">mASC</jats:styled-content>s by up‐regulation of genes including <jats:italic>Osx</jats:italic>,<jats:italic> Runx2</jats:italic>,<jats:italic> BMP‐2</jats:italic>,<jats:italic> Col‐1</jats:italic>,<jats:italic> OPN</jats:italic> and <jats:italic>OCN</jats:italic>.</jats:p></jats:sec> |
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| Umfang: | 124-132 |
| ISSN: |
0960-7722
1365-2184 |
| DOI: | 10.1111/cpr.12097 |