Transplantation of Nurr1‐overexpressing neural stem cells and microglia for treating parkinsonian rats

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Zeitschriftentitel:	CNS Neuroscience & Therapeutics
Personen und Körperschaften:	Qian, Yuan, Chen, Xiao‐Xiang, Wang, Wei, Li, Jun‐Jun, Wang, Xian‐Peng, Tang, Zhi‐Wei, Xu, Jiao‐Tian, Lin, Hai, Yang, Zhi‐Yong, Li, Li‐Yan, Song, Xiao‐Bin, Guo, Jia‐Zhi, Bian, Li‐Gong, Zhou, Lei, Lu, Di, Deng, Xing‐Li
In:	CNS Neuroscience & Therapeutics, 26, 2020, 1, S. 55-65
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Wiley
Schlagwörter:	Pharmacology (medical) Physiology (medical) Psychiatry and Mental health Pharmacology

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Zusammenfassung:	<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Neural stem cells (NSCs) transplantation is considered a promising treatment for Parkinson's disease. But most NSCs are differentiated into glial cells rather than neurons, and only a few of them survive after transplantation due to the inflammatory environment.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>In this study, neural stem cells (NSCs) and microglial cells both forced with the <jats:italic>Nurr1</jats:italic> gene were transplanted into the striatum of the rat model of PD. The results were evaluated through reverse transcription polymerase chain reaction (RT‐PCR), Western blot, and immunofluorescence analysis.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The behavioral abnormalities of PD rats were improved by combined transplantation of NSCs and microglia, both forced with <jats:italic>Nurr1</jats:italic>. The number of tyrosine hydroxylase+ cells in the striatum of PD rats increased, and the number of Iba1+ cells decreased compared with the other groups. Moreover, the dopamine neurons differentiated from grafted NSCs could still be detected in the striatum of PD rats after 5 months.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The results suggested that transplantation of Nurr1‐overexpressing NSCs and microglia could improve the inhospitable host brain environments, which will be a new potential strategy for the cell replacement therapy in PD.</jats:p></jats:sec>
Umfang:	55-65
ISSN:	1755-5930 1755-5949
DOI:	10.1111/cns.13149