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Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens
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| Zeitschriftentitel: | Genome Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , |
| In: | Genome Research, 13, 2003, 7, S. 1572-1579 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
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| Schlagwörter: |
| author_facet |
Nishio, Yousuke Nakamura, Yoji Kawarabayasi, Yutaka Usuda, Yoshihiro Kimura, Eiichiro Sugimoto, Shinichi Matsui, Kazuhiko Yamagishi, Akihiko Kikuchi, Hisashi Ikeo, Kazuho Gojobori, Takashi Nishio, Yousuke Nakamura, Yoji Kawarabayasi, Yutaka Usuda, Yoshihiro Kimura, Eiichiro Sugimoto, Shinichi Matsui, Kazuhiko Yamagishi, Akihiko Kikuchi, Hisashi Ikeo, Kazuho Gojobori, Takashi |
|---|---|
| author |
Nishio, Yousuke Nakamura, Yoji Kawarabayasi, Yutaka Usuda, Yoshihiro Kimura, Eiichiro Sugimoto, Shinichi Matsui, Kazuhiko Yamagishi, Akihiko Kikuchi, Hisashi Ikeo, Kazuho Gojobori, Takashi |
| spellingShingle |
Nishio, Yousuke Nakamura, Yoji Kawarabayasi, Yutaka Usuda, Yoshihiro Kimura, Eiichiro Sugimoto, Shinichi Matsui, Kazuhiko Yamagishi, Akihiko Kikuchi, Hisashi Ikeo, Kazuho Gojobori, Takashi Genome Research Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens Genetics (clinical) Genetics |
| author_sort |
nishio, yousuke |
| spelling |
Nishio, Yousuke Nakamura, Yoji Kawarabayasi, Yutaka Usuda, Yoshihiro Kimura, Eiichiro Sugimoto, Shinichi Matsui, Kazuhiko Yamagishi, Akihiko Kikuchi, Hisashi Ikeo, Kazuho Gojobori, Takashi 1088-9051 Cold Spring Harbor Laboratory Genetics (clinical) Genetics http://dx.doi.org/10.1101/gr.1285603 <jats:p><jats:italic>Corynebacterium efficiens</jats:italic>is the closest relative of<jats:italic>Corynebacterium glutamicum,</jats:italic>a species widely used for the industrial production of amino acids.<jats:italic>C. efficiens</jats:italic>but not<jats:italic>C. glutamicum</jats:italic>can grow above 40°C. We sequenced the complete<jats:italic>C. efficiens</jats:italic>genome to investigate the basis of its thermostability by comparing its genome with that of<jats:italic>C. glutamicum</jats:italic>. The difference in GC content between the species was reflected in codon usage and nucleotide substitutions. Our comparative genomic study clearly showed that there was tremendous bias in amino acid substitutions in all orthologous ORFs. Analysis of the direction of the amino acid substitutions suggested that three substitutions are important for the stability of the<jats:italic>C. efficiens</jats:italic>proteins: from lysine to arginine, serine to alanine, and serine to threonine. Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of<jats:italic>C. efficiens</jats:italic>.</jats:p> Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability of<i>Corynebacterium efficiens</i> Genome Research |
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Cold Spring Harbor Laboratory, 2003 |
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| title |
Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_unstemmed |
Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_full |
Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_fullStr |
Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_full_unstemmed |
Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_short |
Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_sort |
comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of<i>corynebacterium efficiens</i> |
| topic |
Genetics (clinical) Genetics |
| url |
http://dx.doi.org/10.1101/gr.1285603 |
| publishDate |
2003 |
| physical |
1572-1579 |
| description |
<jats:p><jats:italic>Corynebacterium efficiens</jats:italic>is the closest relative of<jats:italic>Corynebacterium glutamicum,</jats:italic>a species widely used for the industrial production of amino acids.<jats:italic>C. efficiens</jats:italic>but not<jats:italic>C. glutamicum</jats:italic>can grow above 40°C. We sequenced the complete<jats:italic>C. efficiens</jats:italic>genome to investigate the basis of its thermostability by comparing its genome with that of<jats:italic>C. glutamicum</jats:italic>. The difference in GC content between the species was reflected in codon usage and nucleotide substitutions. Our comparative genomic study clearly showed that there was tremendous bias in amino acid substitutions in all orthologous ORFs. Analysis of the direction of the amino acid substitutions suggested that three substitutions are important for the stability of the<jats:italic>C. efficiens</jats:italic>proteins: from lysine to arginine, serine to alanine, and serine to threonine. Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of<jats:italic>C. efficiens</jats:italic>.</jats:p> |
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| author | Nishio, Yousuke, Nakamura, Yoji, Kawarabayasi, Yutaka, Usuda, Yoshihiro, Kimura, Eiichiro, Sugimoto, Shinichi, Matsui, Kazuhiko, Yamagishi, Akihiko, Kikuchi, Hisashi, Ikeo, Kazuho, Gojobori, Takashi |
| author_facet | Nishio, Yousuke, Nakamura, Yoji, Kawarabayasi, Yutaka, Usuda, Yoshihiro, Kimura, Eiichiro, Sugimoto, Shinichi, Matsui, Kazuhiko, Yamagishi, Akihiko, Kikuchi, Hisashi, Ikeo, Kazuho, Gojobori, Takashi, Nishio, Yousuke, Nakamura, Yoji, Kawarabayasi, Yutaka, Usuda, Yoshihiro, Kimura, Eiichiro, Sugimoto, Shinichi, Matsui, Kazuhiko, Yamagishi, Akihiko, Kikuchi, Hisashi, Ikeo, Kazuho, Gojobori, Takashi |
| author_sort | nishio, yousuke |
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| description | <jats:p><jats:italic>Corynebacterium efficiens</jats:italic>is the closest relative of<jats:italic>Corynebacterium glutamicum,</jats:italic>a species widely used for the industrial production of amino acids.<jats:italic>C. efficiens</jats:italic>but not<jats:italic>C. glutamicum</jats:italic>can grow above 40°C. We sequenced the complete<jats:italic>C. efficiens</jats:italic>genome to investigate the basis of its thermostability by comparing its genome with that of<jats:italic>C. glutamicum</jats:italic>. The difference in GC content between the species was reflected in codon usage and nucleotide substitutions. Our comparative genomic study clearly showed that there was tremendous bias in amino acid substitutions in all orthologous ORFs. Analysis of the direction of the amino acid substitutions suggested that three substitutions are important for the stability of the<jats:italic>C. efficiens</jats:italic>proteins: from lysine to arginine, serine to alanine, and serine to threonine. Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of<jats:italic>C. efficiens</jats:italic>.</jats:p> |
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| imprint | Cold Spring Harbor Laboratory, 2003 |
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| institution | DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| spelling | Nishio, Yousuke Nakamura, Yoji Kawarabayasi, Yutaka Usuda, Yoshihiro Kimura, Eiichiro Sugimoto, Shinichi Matsui, Kazuhiko Yamagishi, Akihiko Kikuchi, Hisashi Ikeo, Kazuho Gojobori, Takashi 1088-9051 Cold Spring Harbor Laboratory Genetics (clinical) Genetics http://dx.doi.org/10.1101/gr.1285603 <jats:p><jats:italic>Corynebacterium efficiens</jats:italic>is the closest relative of<jats:italic>Corynebacterium glutamicum,</jats:italic>a species widely used for the industrial production of amino acids.<jats:italic>C. efficiens</jats:italic>but not<jats:italic>C. glutamicum</jats:italic>can grow above 40°C. We sequenced the complete<jats:italic>C. efficiens</jats:italic>genome to investigate the basis of its thermostability by comparing its genome with that of<jats:italic>C. glutamicum</jats:italic>. The difference in GC content between the species was reflected in codon usage and nucleotide substitutions. Our comparative genomic study clearly showed that there was tremendous bias in amino acid substitutions in all orthologous ORFs. Analysis of the direction of the amino acid substitutions suggested that three substitutions are important for the stability of the<jats:italic>C. efficiens</jats:italic>proteins: from lysine to arginine, serine to alanine, and serine to threonine. Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of<jats:italic>C. efficiens</jats:italic>.</jats:p> Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability of<i>Corynebacterium efficiens</i> Genome Research |
| spellingShingle | Nishio, Yousuke, Nakamura, Yoji, Kawarabayasi, Yutaka, Usuda, Yoshihiro, Kimura, Eiichiro, Sugimoto, Shinichi, Matsui, Kazuhiko, Yamagishi, Akihiko, Kikuchi, Hisashi, Ikeo, Kazuho, Gojobori, Takashi, Genome Research, Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens, Genetics (clinical), Genetics |
| title | Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_full | Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_fullStr | Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_full_unstemmed | Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_short | Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| title_sort | comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of<i>corynebacterium efficiens</i> |
| title_unstemmed | Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens |
| topic | Genetics (clinical), Genetics |
| url | http://dx.doi.org/10.1101/gr.1285603 |