Comparative Complete Genome Sequence Analysis of the Amino Acid Replacements Responsible for the Thermostability ofCorynebacterium efficiens

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Bibliographische Detailangaben
Zeitschriftentitel:	Genome Research
Personen und Körperschaften:	Nishio, Yousuke, Nakamura, Yoji, Kawarabayasi, Yutaka, Usuda, Yoshihiro, Kimura, Eiichiro, Sugimoto, Shinichi, Matsui, Kazuhiko, Yamagishi, Akihiko, Kikuchi, Hisashi, Ikeo, Kazuho, Gojobori, Takashi
In:	Genome Research, 13, 2003, 7, S. 1572-1579
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Cold Spring Harbor Laboratory
Schlagwörter:	Genetics (clinical) Genetics

Details
Zusammenfassung:	<jats:p><jats:italic>Corynebacterium efficiens</jats:italic>is the closest relative of<jats:italic>Corynebacterium glutamicum,</jats:italic>a species widely used for the industrial production of amino acids.<jats:italic>C. efficiens</jats:italic>but not<jats:italic>C. glutamicum</jats:italic>can grow above 40°C. We sequenced the complete<jats:italic>C. efficiens</jats:italic>genome to investigate the basis of its thermostability by comparing its genome with that of<jats:italic>C. glutamicum</jats:italic>. The difference in GC content between the species was reflected in codon usage and nucleotide substitutions. Our comparative genomic study clearly showed that there was tremendous bias in amino acid substitutions in all orthologous ORFs. Analysis of the direction of the amino acid substitutions suggested that three substitutions are important for the stability of the<jats:italic>C. efficiens</jats:italic>proteins: from lysine to arginine, serine to alanine, and serine to threonine. Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of<jats:italic>C. efficiens</jats:italic>.</jats:p>
Umfang:	1572-1579
ISSN:	1088-9051
DOI:	10.1101/gr.1285603