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Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells
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| Zeitschriftentitel: | Molecular Cytogenetics |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , |
| In: | Molecular Cytogenetics, 2, 2009, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
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| Schlagwörter: |
| author_facet |
Erlecke, Jörn Hartmann, Isabell Hoffmann, Martin Kroll, Torsten Starke, Heike Heller, Anita Gloria, Alexander Sayer, Herbert G Johannes, Tilman Claussen, Uwe Liehr, Thomas Loncarevic, Ivan F Erlecke, Jörn Hartmann, Isabell Hoffmann, Martin Kroll, Torsten Starke, Heike Heller, Anita Gloria, Alexander Sayer, Herbert G Johannes, Tilman Claussen, Uwe Liehr, Thomas Loncarevic, Ivan F |
|---|---|
| author |
Erlecke, Jörn Hartmann, Isabell Hoffmann, Martin Kroll, Torsten Starke, Heike Heller, Anita Gloria, Alexander Sayer, Herbert G Johannes, Tilman Claussen, Uwe Liehr, Thomas Loncarevic, Ivan F |
| spellingShingle |
Erlecke, Jörn Hartmann, Isabell Hoffmann, Martin Kroll, Torsten Starke, Heike Heller, Anita Gloria, Alexander Sayer, Herbert G Johannes, Tilman Claussen, Uwe Liehr, Thomas Loncarevic, Ivan F Molecular Cytogenetics Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells Biochemistry (medical) Genetics (clinical) Genetics Molecular Biology Molecular Medicine Biochemistry |
| author_sort |
erlecke, jörn |
| spelling |
Erlecke, Jörn Hartmann, Isabell Hoffmann, Martin Kroll, Torsten Starke, Heike Heller, Anita Gloria, Alexander Sayer, Herbert G Johannes, Tilman Claussen, Uwe Liehr, Thomas Loncarevic, Ivan F 1755-8166 Springer Science and Business Media LLC Biochemistry (medical) Genetics (clinical) Genetics Molecular Biology Molecular Medicine Biochemistry http://dx.doi.org/10.1186/1755-8166-2-12 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.</jats:p> <jats:p>Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1–1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.</jats:p> </jats:sec> Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells Molecular Cytogenetics |
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10.1186/1755-8166-2-12 |
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Biologie Medizin Chemie und Pharmazie |
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Springer Science and Business Media LLC, 2009 |
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Springer Science and Business Media LLC, 2009 |
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2009 |
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Molecular Cytogenetics |
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| title |
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_unstemmed |
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_full |
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_fullStr |
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_full_unstemmed |
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_short |
Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_sort |
automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| topic |
Biochemistry (medical) Genetics (clinical) Genetics Molecular Biology Molecular Medicine Biochemistry |
| url |
http://dx.doi.org/10.1186/1755-8166-2-12 |
| publishDate |
2009 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.</jats:p>
<jats:p>Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1–1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusion</jats:title>
<jats:p>The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.</jats:p>
</jats:sec> |
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| author | Erlecke, Jörn, Hartmann, Isabell, Hoffmann, Martin, Kroll, Torsten, Starke, Heike, Heller, Anita, Gloria, Alexander, Sayer, Herbert G, Johannes, Tilman, Claussen, Uwe, Liehr, Thomas, Loncarevic, Ivan F |
| author_facet | Erlecke, Jörn, Hartmann, Isabell, Hoffmann, Martin, Kroll, Torsten, Starke, Heike, Heller, Anita, Gloria, Alexander, Sayer, Herbert G, Johannes, Tilman, Claussen, Uwe, Liehr, Thomas, Loncarevic, Ivan F, Erlecke, Jörn, Hartmann, Isabell, Hoffmann, Martin, Kroll, Torsten, Starke, Heike, Heller, Anita, Gloria, Alexander, Sayer, Herbert G, Johannes, Tilman, Claussen, Uwe, Liehr, Thomas, Loncarevic, Ivan F |
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| description | <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.</jats:p> <jats:p>Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1–1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.</jats:p> </jats:sec> |
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| imprint | Springer Science and Business Media LLC, 2009 |
| imprint_str_mv | Springer Science and Business Media LLC, 2009 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Erlecke, Jörn Hartmann, Isabell Hoffmann, Martin Kroll, Torsten Starke, Heike Heller, Anita Gloria, Alexander Sayer, Herbert G Johannes, Tilman Claussen, Uwe Liehr, Thomas Loncarevic, Ivan F 1755-8166 Springer Science and Business Media LLC Biochemistry (medical) Genetics (clinical) Genetics Molecular Biology Molecular Medicine Biochemistry http://dx.doi.org/10.1186/1755-8166-2-12 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.</jats:p> <jats:p>Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1–1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.</jats:p> </jats:sec> Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells Molecular Cytogenetics |
| spellingShingle | Erlecke, Jörn, Hartmann, Isabell, Hoffmann, Martin, Kroll, Torsten, Starke, Heike, Heller, Anita, Gloria, Alexander, Sayer, Herbert G, Johannes, Tilman, Claussen, Uwe, Liehr, Thomas, Loncarevic, Ivan F, Molecular Cytogenetics, Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells, Biochemistry (medical), Genetics (clinical), Genetics, Molecular Biology, Molecular Medicine, Biochemistry |
| title | Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_full | Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_fullStr | Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_full_unstemmed | Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_short | Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_sort | automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| title_unstemmed | Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells |
| topic | Biochemistry (medical), Genetics (clinical), Genetics, Molecular Biology, Molecular Medicine, Biochemistry |
| url | http://dx.doi.org/10.1186/1755-8166-2-12 |