Automated detection of residual cells after sex-mismatched stem-cell transplantation – evidence for presence of disease-marker negative residual cells

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Zeitschriftentitel:	Molecular Cytogenetics
Personen und Körperschaften:	Erlecke, Jörn, Hartmann, Isabell, Hoffmann, Martin, Kroll, Torsten, Starke, Heike, Heller, Anita, Gloria, Alexander, Sayer, Herbert G, Johannes, Tilman, Claussen, Uwe, Liehr, Thomas, Loncarevic, Ivan F
In:	Molecular Cytogenetics, 2, 2009, 1
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Springer Science and Business Media LLC
Schlagwörter:	Biochemistry (medical) Genetics (clinical) Genetics Molecular Biology Molecular Medicine Biochemistry

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Zusammenfassung:	<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.</jats:p> <jats:p>Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1–1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.</jats:p> </jats:sec>
ISSN:	1755-8166
DOI:	10.1186/1755-8166-2-12