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Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis
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| Zeitschriftentitel: | Journal of International Medical Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Journal of International Medical Research, 37, 2009, 2, S. 417-425 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Wang, W Xu, G-L Jia, W-D Wang, Z-H Li, J-S Ma, J-L Ge, Y-S Xie, S-X Yu, J-H Wang, W Xu, G-L Jia, W-D Wang, Z-H Li, J-S Ma, J-L Ge, Y-S Xie, S-X Yu, J-H |
|---|---|
| author |
Wang, W Xu, G-L Jia, W-D Wang, Z-H Li, J-S Ma, J-L Ge, Y-S Xie, S-X Yu, J-H |
| spellingShingle |
Wang, W Xu, G-L Jia, W-D Wang, Z-H Li, J-S Ma, J-L Ge, Y-S Xie, S-X Yu, J-H Journal of International Medical Research Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis Biochemistry (medical) Cell Biology Biochemistry General Medicine |
| author_sort |
wang, w |
| spelling |
Wang, W Xu, G-L Jia, W-D Wang, Z-H Li, J-S Ma, J-L Ge, Y-S Xie, S-X Yu, J-H 0300-0605 1473-2300 SAGE Publications Biochemistry (medical) Cell Biology Biochemistry General Medicine http://dx.doi.org/10.1177/147323000903700217 <jats:p> An experimental rat hepatocellular carcinoma (HCC) model was established using diethylnitrosamine and N-nitrosomorpholine to induce carcinogenesis in Sprague-Dawley rats. During hepatocarcinogenesis, seven rats were sacrificed at 0, 4, 8, 12 and 16 weeks and 10 rats were sacrificed at 20 weeks. The levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) protein and mRNA were examined by immunohistochemistry, Western blot and semi-quantitative reverse transcriptase-polymerase chain reaction at different stages in the rat HCC model. Twenty weeks after induction of hepatocarcinogenesis, the expression of HIF-1α and VEGF protein and mRNA significantly increased compared with week 0. Microvessel density (MVD) increased considerably once liver cancer developed. There was a significant positive correlation between MVD and both HIF-1α and VEGF, and between HIF-1α and VEGF levels. These results suggest that HIF-1α and VEGF play important roles in tumour occurrence and development during rat hepatocarcinogenesis, possibly through promoting tumour angiogenesis. </jats:p> Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis Journal of International Medical Research |
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SAGE Publications, 2009 |
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| title |
Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_unstemmed |
Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_full |
Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_fullStr |
Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_full_unstemmed |
Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_short |
Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_sort |
expression and correlation of hypoxia-inducible factor-1α, vascular endothelial growth factor and microvessel density in experimental rat hepatocarcinogenesis |
| topic |
Biochemistry (medical) Cell Biology Biochemistry General Medicine |
| url |
http://dx.doi.org/10.1177/147323000903700217 |
| publishDate |
2009 |
| physical |
417-425 |
| description |
<jats:p> An experimental rat hepatocellular carcinoma (HCC) model was established using diethylnitrosamine and N-nitrosomorpholine to induce carcinogenesis in Sprague-Dawley rats. During hepatocarcinogenesis, seven rats were sacrificed at 0, 4, 8, 12 and 16 weeks and 10 rats were sacrificed at 20 weeks. The levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) protein and mRNA were examined by immunohistochemistry, Western blot and semi-quantitative reverse transcriptase-polymerase chain reaction at different stages in the rat HCC model. Twenty weeks after induction of hepatocarcinogenesis, the expression of HIF-1α and VEGF protein and mRNA significantly increased compared with week 0. Microvessel density (MVD) increased considerably once liver cancer developed. There was a significant positive correlation between MVD and both HIF-1α and VEGF, and between HIF-1α and VEGF levels. These results suggest that HIF-1α and VEGF play important roles in tumour occurrence and development during rat hepatocarcinogenesis, possibly through promoting tumour angiogenesis. </jats:p> |
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| author | Wang, W, Xu, G-L, Jia, W-D, Wang, Z-H, Li, J-S, Ma, J-L, Ge, Y-S, Xie, S-X, Yu, J-H |
| author_facet | Wang, W, Xu, G-L, Jia, W-D, Wang, Z-H, Li, J-S, Ma, J-L, Ge, Y-S, Xie, S-X, Yu, J-H, Wang, W, Xu, G-L, Jia, W-D, Wang, Z-H, Li, J-S, Ma, J-L, Ge, Y-S, Xie, S-X, Yu, J-H |
| author_sort | wang, w |
| container_issue | 2 |
| container_start_page | 417 |
| container_title | Journal of International Medical Research |
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| description | <jats:p> An experimental rat hepatocellular carcinoma (HCC) model was established using diethylnitrosamine and N-nitrosomorpholine to induce carcinogenesis in Sprague-Dawley rats. During hepatocarcinogenesis, seven rats were sacrificed at 0, 4, 8, 12 and 16 weeks and 10 rats were sacrificed at 20 weeks. The levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) protein and mRNA were examined by immunohistochemistry, Western blot and semi-quantitative reverse transcriptase-polymerase chain reaction at different stages in the rat HCC model. Twenty weeks after induction of hepatocarcinogenesis, the expression of HIF-1α and VEGF protein and mRNA significantly increased compared with week 0. Microvessel density (MVD) increased considerably once liver cancer developed. There was a significant positive correlation between MVD and both HIF-1α and VEGF, and between HIF-1α and VEGF levels. These results suggest that HIF-1α and VEGF play important roles in tumour occurrence and development during rat hepatocarcinogenesis, possibly through promoting tumour angiogenesis. </jats:p> |
| doi_str_mv | 10.1177/147323000903700217 |
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| imprint | SAGE Publications, 2009 |
| imprint_str_mv | SAGE Publications, 2009 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| issn_str_mv | 1473-2300, 0300-0605 |
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| mega_collection | SAGE Publications (CrossRef) |
| physical | 417-425 |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SAGE Publications |
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| series | Journal of International Medical Research |
| source_id | 49 |
| spelling | Wang, W Xu, G-L Jia, W-D Wang, Z-H Li, J-S Ma, J-L Ge, Y-S Xie, S-X Yu, J-H 0300-0605 1473-2300 SAGE Publications Biochemistry (medical) Cell Biology Biochemistry General Medicine http://dx.doi.org/10.1177/147323000903700217 <jats:p> An experimental rat hepatocellular carcinoma (HCC) model was established using diethylnitrosamine and N-nitrosomorpholine to induce carcinogenesis in Sprague-Dawley rats. During hepatocarcinogenesis, seven rats were sacrificed at 0, 4, 8, 12 and 16 weeks and 10 rats were sacrificed at 20 weeks. The levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) protein and mRNA were examined by immunohistochemistry, Western blot and semi-quantitative reverse transcriptase-polymerase chain reaction at different stages in the rat HCC model. Twenty weeks after induction of hepatocarcinogenesis, the expression of HIF-1α and VEGF protein and mRNA significantly increased compared with week 0. Microvessel density (MVD) increased considerably once liver cancer developed. There was a significant positive correlation between MVD and both HIF-1α and VEGF, and between HIF-1α and VEGF levels. These results suggest that HIF-1α and VEGF play important roles in tumour occurrence and development during rat hepatocarcinogenesis, possibly through promoting tumour angiogenesis. </jats:p> Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis Journal of International Medical Research |
| spellingShingle | Wang, W, Xu, G-L, Jia, W-D, Wang, Z-H, Li, J-S, Ma, J-L, Ge, Y-S, Xie, S-X, Yu, J-H, Journal of International Medical Research, Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis, Biochemistry (medical), Cell Biology, Biochemistry, General Medicine |
| title | Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_full | Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_fullStr | Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_full_unstemmed | Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_short | Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| title_sort | expression and correlation of hypoxia-inducible factor-1α, vascular endothelial growth factor and microvessel density in experimental rat hepatocarcinogenesis |
| title_unstemmed | Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis |
| topic | Biochemistry (medical), Cell Biology, Biochemistry, General Medicine |
| url | http://dx.doi.org/10.1177/147323000903700217 |