Expression and Correlation of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor and Microvessel Density in Experimental Rat Hepatocarcinogenesis

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Bibliographische Detailangaben
Zeitschriftentitel:	Journal of International Medical Research
Personen und Körperschaften:	Wang, W, Xu, G-L, Jia, W-D, Wang, Z-H, Li, J-S, Ma, J-L, Ge, Y-S, Xie, S-X, Yu, J-H
In:	Journal of International Medical Research, 37, 2009, 2, S. 417-425
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	SAGE Publications
Schlagwörter:	Biochemistry (medical) Cell Biology Biochemistry General Medicine

Details
Zusammenfassung:	<jats:p> An experimental rat hepatocellular carcinoma (HCC) model was established using diethylnitrosamine and N-nitrosomorpholine to induce carcinogenesis in Sprague-Dawley rats. During hepatocarcinogenesis, seven rats were sacrificed at 0, 4, 8, 12 and 16 weeks and 10 rats were sacrificed at 20 weeks. The levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) protein and mRNA were examined by immunohistochemistry, Western blot and semi-quantitative reverse transcriptase-polymerase chain reaction at different stages in the rat HCC model. Twenty weeks after induction of hepatocarcinogenesis, the expression of HIF-1α and VEGF protein and mRNA significantly increased compared with week 0. Microvessel density (MVD) increased considerably once liver cancer developed. There was a significant positive correlation between MVD and both HIF-1α and VEGF, and between HIF-1α and VEGF levels. These results suggest that HIF-1α and VEGF play important roles in tumour occurrence and development during rat hepatocarcinogenesis, possibly through promoting tumour angiogenesis. </jats:p>
Umfang:	417-425
ISSN:	1473-2300 0300-0605
DOI:	10.1177/147323000903700217