Jmjd3 Controls Mesodermal and Cardiovascular Differentiation of Embryonic Stem Cells

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Bibliographische Detailangaben
Zeitschriftentitel:	Circulation Research
Personen und Körperschaften:	Ohtani, Kisho, Zhao, Cong, Dobreva, Gergana, Manavski, Yosif, Kluge, Britta, Braun, Thomas, Rieger, Michael A., Zeiher, Andreas M., Dimmeler, Stefanie
In:	Circulation Research, 113, 2013, 7, S. 856-862
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Ovid Technologies (Wolters Kluwer Health)
Schlagwörter:	Cardiology and Cardiovascular Medicine Physiology

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Zusammenfassung:	<jats:sec> <jats:title> <jats:underline>Rationale:</jats:underline> </jats:title> <jats:p>The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear.</jats:p> </jats:sec> <jats:sec> <jats:title> <jats:underline>Objective:</jats:underline> </jats:title> <jats:p>To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment.</jats:p> </jats:sec> <jats:sec> <jats:title> <jats:underline>Methods and Results:</jats:underline> </jats:title> <jats:p> Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the <jats:italic>Brachyury</jats:italic> promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal–induced mesoderm differentiation. </jats:p> </jats:sec> <jats:sec> <jats:title> <jats:underline>Conclusions:</jats:underline> </jats:title> <jats:p>These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.</jats:p> </jats:sec>
Umfang:	856-862
ISSN:	0009-7330 1524-4571
DOI:	10.1161/circresaha.113.302035