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EGFRAmplification and Glioblastoma Stem-Like Cells
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| Zeitschriftentitel: | Stem Cells International |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Stem Cells International, 2015, 2015, S. 1-11 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
|
| Schlagwörter: |
| author_facet |
Liffers, Katrin Lamszus, Katrin Schulte, Alexander Liffers, Katrin Lamszus, Katrin Schulte, Alexander |
|---|---|
| author |
Liffers, Katrin Lamszus, Katrin Schulte, Alexander |
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Liffers, Katrin Lamszus, Katrin Schulte, Alexander Stem Cells International EGFRAmplification and Glioblastoma Stem-Like Cells Cell Biology Molecular Biology |
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liffers, katrin |
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Liffers, Katrin Lamszus, Katrin Schulte, Alexander 1687-966X 1687-9678 Hindawi Limited Cell Biology Molecular Biology http://dx.doi.org/10.1155/2015/427518 <jats:p>Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained<jats:italic>in vitro</jats:italic>using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (<jats:italic>EGFR</jats:italic>) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained<jats:italic>EGFR</jats:italic>amplification could overcome the limitations of current<jats:italic>in vitro</jats:italic>model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different<jats:italic>EGFR</jats:italic>status in order to maintain EGFR-dependent intratumoral heterogeneity<jats:italic>in vitro</jats:italic>. Further, it will summarize the current knowledge about the impact of<jats:italic>EGFR</jats:italic>amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM.</jats:p> <i>EGFR</i>Amplification and Glioblastoma Stem-Like Cells Stem Cells International |
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| title |
EGFRAmplification and Glioblastoma Stem-Like Cells |
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EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_full |
EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_fullStr |
EGFRAmplification and Glioblastoma Stem-Like Cells |
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EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_short |
EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_sort |
<i>egfr</i>amplification and glioblastoma stem-like cells |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1155/2015/427518 |
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2015 |
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1-11 |
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<jats:p>Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained<jats:italic>in vitro</jats:italic>using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (<jats:italic>EGFR</jats:italic>) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained<jats:italic>EGFR</jats:italic>amplification could overcome the limitations of current<jats:italic>in vitro</jats:italic>model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different<jats:italic>EGFR</jats:italic>status in order to maintain EGFR-dependent intratumoral heterogeneity<jats:italic>in vitro</jats:italic>. Further, it will summarize the current knowledge about the impact of<jats:italic>EGFR</jats:italic>amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM.</jats:p> |
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| author | Liffers, Katrin, Lamszus, Katrin, Schulte, Alexander |
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| description | <jats:p>Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained<jats:italic>in vitro</jats:italic>using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (<jats:italic>EGFR</jats:italic>) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained<jats:italic>EGFR</jats:italic>amplification could overcome the limitations of current<jats:italic>in vitro</jats:italic>model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different<jats:italic>EGFR</jats:italic>status in order to maintain EGFR-dependent intratumoral heterogeneity<jats:italic>in vitro</jats:italic>. Further, it will summarize the current knowledge about the impact of<jats:italic>EGFR</jats:italic>amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM.</jats:p> |
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| spelling | Liffers, Katrin Lamszus, Katrin Schulte, Alexander 1687-966X 1687-9678 Hindawi Limited Cell Biology Molecular Biology http://dx.doi.org/10.1155/2015/427518 <jats:p>Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained<jats:italic>in vitro</jats:italic>using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (<jats:italic>EGFR</jats:italic>) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained<jats:italic>EGFR</jats:italic>amplification could overcome the limitations of current<jats:italic>in vitro</jats:italic>model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different<jats:italic>EGFR</jats:italic>status in order to maintain EGFR-dependent intratumoral heterogeneity<jats:italic>in vitro</jats:italic>. Further, it will summarize the current knowledge about the impact of<jats:italic>EGFR</jats:italic>amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM.</jats:p> <i>EGFR</i>Amplification and Glioblastoma Stem-Like Cells Stem Cells International |
| spellingShingle | Liffers, Katrin, Lamszus, Katrin, Schulte, Alexander, Stem Cells International, EGFRAmplification and Glioblastoma Stem-Like Cells, Cell Biology, Molecular Biology |
| title | EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_full | EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_fullStr | EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_full_unstemmed | EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_short | EGFRAmplification and Glioblastoma Stem-Like Cells |
| title_sort | <i>egfr</i>amplification and glioblastoma stem-like cells |
| title_unstemmed | EGFRAmplification and Glioblastoma Stem-Like Cells |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1155/2015/427518 |