Eintrag weiter verarbeiten
EGFRAmplification and Glioblastoma Stem-Like Cells
Gespeichert in:
| Zeitschriftentitel: | Stem Cells International |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Stem Cells International, 2015, 2015, S. 1-11 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
|
| Schlagwörter: |
| Zusammenfassung: | <jats:p>Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained<jats:italic>in vitro</jats:italic>using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (<jats:italic>EGFR</jats:italic>) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained<jats:italic>EGFR</jats:italic>amplification could overcome the limitations of current<jats:italic>in vitro</jats:italic>model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different<jats:italic>EGFR</jats:italic>status in order to maintain EGFR-dependent intratumoral heterogeneity<jats:italic>in vitro</jats:italic>. Further, it will summarize the current knowledge about the impact of<jats:italic>EGFR</jats:italic>amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM.</jats:p> |
|---|---|
| Umfang: | 1-11 |
| ISSN: |
1687-966X
1687-9678 |
| DOI: | 10.1155/2015/427518 |