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Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae
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| Zeitschriftentitel: | Allergy |
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| Personen und Körperschaften: | , , , , |
| In: | Allergy, 57, 2002, 8, S. 729-731 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| Zusammenfassung: | <jats:p> <jats:bold>Background: </jats:bold> House dust mite derived materials are known to be the most potent agent inducing allergic diseases. Localization of Der f 2 was attempted to specify the sites and concentrations of Der f 2 within the mite, which may indicate the importance of secreted materials and nonexcreted body components as allergen sources.</jats:p><jats:p> <jats:bold>Methods: </jats:bold> Serial cryostat sections of embedded live mites and the fecal pellets, collected by brush, were immunoprobed using monoclonal antibody (mAb) 2F38 raised against recombinant (r) Der f 2.</jats:p><jats:p> <jats:bold>Results: </jats:bold> Highest concentrations were found in the anterior midgut, implying that this is the site of Der f 2 synthesis and secretion. Digestive material and defecated fecal pellets were also labeled with mAb.</jats:p><jats:p> <jats:bold>Conclusions: </jats:bold> Our results show that the major allergen, Der f 2, found in the house dust mite <jats:italic>D. farinae</jats:italic> is derived from the digestive tract, and is concentrated in the feces.</jats:p> |
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| Umfang: | 729-731 |
| ISSN: |
0105-4538
1398-9995 |
| DOI: | 10.1034/j.1398-9995.2002.23623.x |