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Long‐lasting RNAi activity in mammalian neurons
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| Zeitschriftentitel: | FEBS Letters |
|---|---|
| Personen und Körperschaften: | , , |
| In: | FEBS Letters, 558, 2004, 1-3, S. 89-95 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Omi, Kazuya Tokunaga, Katsushi Hohjoh, Hirohiko Omi, Kazuya Tokunaga, Katsushi Hohjoh, Hirohiko |
|---|---|
| author |
Omi, Kazuya Tokunaga, Katsushi Hohjoh, Hirohiko |
| spellingShingle |
Omi, Kazuya Tokunaga, Katsushi Hohjoh, Hirohiko FEBS Letters Long‐lasting RNAi activity in mammalian neurons Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
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omi, kazuya |
| spelling |
Omi, Kazuya Tokunaga, Katsushi Hohjoh, Hirohiko 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1016/s0014-5793(04)00017-1 <jats:p>The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21‐nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long‐lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence‐specific RNAi activity involving long dsRNA appears to be masked.</jats:p> Long‐lasting RNAi activity in mammalian neurons FEBS Letters |
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10.1016/s0014-5793(04)00017-1 |
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Wiley, 2004 |
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Wiley, 2004 |
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0014-5793 1873-3468 |
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0014-5793 1873-3468 |
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2004 |
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Wiley |
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FEBS Letters |
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49 |
| title |
Long‐lasting RNAi activity in mammalian neurons |
| title_unstemmed |
Long‐lasting RNAi activity in mammalian neurons |
| title_full |
Long‐lasting RNAi activity in mammalian neurons |
| title_fullStr |
Long‐lasting RNAi activity in mammalian neurons |
| title_full_unstemmed |
Long‐lasting RNAi activity in mammalian neurons |
| title_short |
Long‐lasting RNAi activity in mammalian neurons |
| title_sort |
long‐lasting rnai activity in mammalian neurons |
| topic |
Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| url |
http://dx.doi.org/10.1016/s0014-5793(04)00017-1 |
| publishDate |
2004 |
| physical |
89-95 |
| description |
<jats:p>The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21‐nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long‐lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence‐specific RNAi activity involving long dsRNA appears to be masked.</jats:p> |
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| author | Omi, Kazuya, Tokunaga, Katsushi, Hohjoh, Hirohiko |
| author_facet | Omi, Kazuya, Tokunaga, Katsushi, Hohjoh, Hirohiko, Omi, Kazuya, Tokunaga, Katsushi, Hohjoh, Hirohiko |
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| container_start_page | 89 |
| container_title | FEBS Letters |
| container_volume | 558 |
| description | <jats:p>The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21‐nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long‐lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence‐specific RNAi activity involving long dsRNA appears to be masked.</jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAxNi9zMDAxNC01NzkzKDA0KTAwMDE3LTE |
| imprint | Wiley, 2004 |
| imprint_str_mv | Wiley, 2004 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 89-95 |
| publishDate | 2004 |
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| recordtype | ai |
| series | FEBS Letters |
| source_id | 49 |
| spelling | Omi, Kazuya Tokunaga, Katsushi Hohjoh, Hirohiko 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1016/s0014-5793(04)00017-1 <jats:p>The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21‐nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long‐lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence‐specific RNAi activity involving long dsRNA appears to be masked.</jats:p> Long‐lasting RNAi activity in mammalian neurons FEBS Letters |
| spellingShingle | Omi, Kazuya, Tokunaga, Katsushi, Hohjoh, Hirohiko, FEBS Letters, Long‐lasting RNAi activity in mammalian neurons, Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| title | Long‐lasting RNAi activity in mammalian neurons |
| title_full | Long‐lasting RNAi activity in mammalian neurons |
| title_fullStr | Long‐lasting RNAi activity in mammalian neurons |
| title_full_unstemmed | Long‐lasting RNAi activity in mammalian neurons |
| title_short | Long‐lasting RNAi activity in mammalian neurons |
| title_sort | long‐lasting rnai activity in mammalian neurons |
| title_unstemmed | Long‐lasting RNAi activity in mammalian neurons |
| topic | Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| url | http://dx.doi.org/10.1016/s0014-5793(04)00017-1 |