Long‐lasting RNAi activity in mammalian neurons

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Bibliographische Detailangaben
Zeitschriftentitel:	FEBS Letters
Personen und Körperschaften:	Omi, Kazuya, Tokunaga, Katsushi, Hohjoh, Hirohiko
In:	FEBS Letters, 558, 2004, 1-3, S. 89-95
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Wiley
Schlagwörter:	Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics

Details
Zusammenfassung:	<jats:p>The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3–7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21‐nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long‐lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence‐specific RNAi activity involving long dsRNA appears to be masked.</jats:p>
Umfang:	89-95
ISSN:	0014-5793 1873-3468
DOI:	10.1016/s0014-5793(04)00017-1