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Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters
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| Zeitschriftentitel: | Developmental Dynamics |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Developmental Dynamics, 234, 2005, 2, S. 387-392 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Zeng, Zhiqiang Liu, Xingjun Seebah, Shalin Gong, Zhiyuan Zeng, Zhiqiang Liu, Xingjun Seebah, Shalin Gong, Zhiyuan |
|---|---|
| author |
Zeng, Zhiqiang Liu, Xingjun Seebah, Shalin Gong, Zhiyuan |
| spellingShingle |
Zeng, Zhiqiang Liu, Xingjun Seebah, Shalin Gong, Zhiyuan Developmental Dynamics Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters Developmental Biology |
| author_sort |
zeng, zhiqiang |
| spelling |
Zeng, Zhiqiang Liu, Xingjun Seebah, Shalin Gong, Zhiyuan 1058-8388 1097-0177 Wiley Developmental Biology http://dx.doi.org/10.1002/dvdy.20491 <jats:title>Abstract</jats:title><jats:p>To test tissue specificity of zebrafish gene promoters in a heterologous fish species, two transgenic medaka lines under two zebrafish promoters were generated. Under the zebrafish skeletal muscle‐specific <jats:italic>mylz2</jats:italic> promoter, transgenic medaka expressed green fluorescent protein (GFP) exclusively in skeletal muscles, mimicking the endogenous medaka <jats:italic>mylz2</jats:italic> mRNA expression and also identical to GFP expression in <jats:italic>mylz2</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. A madaka <jats:italic>mylz2</jats:italic> promoter was also capable of directing skeletal muscle‐specific GFP expression in transient transgenic zebrafish embryos. In the <jats:italic>krt8</jats:italic>:<jats:italic>rfp</jats:italic> transgenic medaka line with the zebrafish epithelial <jats:italic>krt8</jats:italic> promoter, red fluorescent protein was specifically expressed in the skin epithelia as well as the epithelial lining cells of the anterior digestive tract, which was also identical to GFP expression in <jats:italic>krt8</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. Therefore, the two zebrafish promoters faithfully function in a heterologous fish species, and it is likely that the mechanisms of tissue‐specific expression are largely conserved among fish species. Developmental Dynamics 234:387–392, 2005. © 2005 Wiley‐Liss, Inc.</jats:p> Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters Developmental Dynamics |
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| title |
Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_unstemmed |
Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_full |
Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_fullStr |
Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_full_unstemmed |
Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_short |
Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_sort |
faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| topic |
Developmental Biology |
| url |
http://dx.doi.org/10.1002/dvdy.20491 |
| publishDate |
2005 |
| physical |
387-392 |
| description |
<jats:title>Abstract</jats:title><jats:p>To test tissue specificity of zebrafish gene promoters in a heterologous fish species, two transgenic medaka lines under two zebrafish promoters were generated. Under the zebrafish skeletal muscle‐specific <jats:italic>mylz2</jats:italic> promoter, transgenic medaka expressed green fluorescent protein (GFP) exclusively in skeletal muscles, mimicking the endogenous medaka <jats:italic>mylz2</jats:italic> mRNA expression and also identical to GFP expression in <jats:italic>mylz2</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. A madaka <jats:italic>mylz2</jats:italic> promoter was also capable of directing skeletal muscle‐specific GFP expression in transient transgenic zebrafish embryos. In the <jats:italic>krt8</jats:italic>:<jats:italic>rfp</jats:italic> transgenic medaka line with the zebrafish epithelial <jats:italic>krt8</jats:italic> promoter, red fluorescent protein was specifically expressed in the skin epithelia as well as the epithelial lining cells of the anterior digestive tract, which was also identical to GFP expression in <jats:italic>krt8</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. Therefore, the two zebrafish promoters faithfully function in a heterologous fish species, and it is likely that the mechanisms of tissue‐specific expression are largely conserved among fish species. Developmental Dynamics 234:387–392, 2005. © 2005 Wiley‐Liss, Inc.</jats:p> |
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| author | Zeng, Zhiqiang, Liu, Xingjun, Seebah, Shalin, Gong, Zhiyuan |
| author_facet | Zeng, Zhiqiang, Liu, Xingjun, Seebah, Shalin, Gong, Zhiyuan, Zeng, Zhiqiang, Liu, Xingjun, Seebah, Shalin, Gong, Zhiyuan |
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| description | <jats:title>Abstract</jats:title><jats:p>To test tissue specificity of zebrafish gene promoters in a heterologous fish species, two transgenic medaka lines under two zebrafish promoters were generated. Under the zebrafish skeletal muscle‐specific <jats:italic>mylz2</jats:italic> promoter, transgenic medaka expressed green fluorescent protein (GFP) exclusively in skeletal muscles, mimicking the endogenous medaka <jats:italic>mylz2</jats:italic> mRNA expression and also identical to GFP expression in <jats:italic>mylz2</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. A madaka <jats:italic>mylz2</jats:italic> promoter was also capable of directing skeletal muscle‐specific GFP expression in transient transgenic zebrafish embryos. In the <jats:italic>krt8</jats:italic>:<jats:italic>rfp</jats:italic> transgenic medaka line with the zebrafish epithelial <jats:italic>krt8</jats:italic> promoter, red fluorescent protein was specifically expressed in the skin epithelia as well as the epithelial lining cells of the anterior digestive tract, which was also identical to GFP expression in <jats:italic>krt8</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. Therefore, the two zebrafish promoters faithfully function in a heterologous fish species, and it is likely that the mechanisms of tissue‐specific expression are largely conserved among fish species. Developmental Dynamics 234:387–392, 2005. © 2005 Wiley‐Liss, Inc.</jats:p> |
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| spelling | Zeng, Zhiqiang Liu, Xingjun Seebah, Shalin Gong, Zhiyuan 1058-8388 1097-0177 Wiley Developmental Biology http://dx.doi.org/10.1002/dvdy.20491 <jats:title>Abstract</jats:title><jats:p>To test tissue specificity of zebrafish gene promoters in a heterologous fish species, two transgenic medaka lines under two zebrafish promoters were generated. Under the zebrafish skeletal muscle‐specific <jats:italic>mylz2</jats:italic> promoter, transgenic medaka expressed green fluorescent protein (GFP) exclusively in skeletal muscles, mimicking the endogenous medaka <jats:italic>mylz2</jats:italic> mRNA expression and also identical to GFP expression in <jats:italic>mylz2</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. A madaka <jats:italic>mylz2</jats:italic> promoter was also capable of directing skeletal muscle‐specific GFP expression in transient transgenic zebrafish embryos. In the <jats:italic>krt8</jats:italic>:<jats:italic>rfp</jats:italic> transgenic medaka line with the zebrafish epithelial <jats:italic>krt8</jats:italic> promoter, red fluorescent protein was specifically expressed in the skin epithelia as well as the epithelial lining cells of the anterior digestive tract, which was also identical to GFP expression in <jats:italic>krt8</jats:italic>:<jats:italic>gfp</jats:italic> transgenic zebrafish. Therefore, the two zebrafish promoters faithfully function in a heterologous fish species, and it is likely that the mechanisms of tissue‐specific expression are largely conserved among fish species. Developmental Dynamics 234:387–392, 2005. © 2005 Wiley‐Liss, Inc.</jats:p> Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters Developmental Dynamics |
| spellingShingle | Zeng, Zhiqiang, Liu, Xingjun, Seebah, Shalin, Gong, Zhiyuan, Developmental Dynamics, Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters, Developmental Biology |
| title | Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_full | Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_fullStr | Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_full_unstemmed | Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_short | Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_sort | faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| title_unstemmed | Faithful expression of living color reporter genes in transgenic medaka under two tissue‐specific zebrafish promoters |
| topic | Developmental Biology |
| url | http://dx.doi.org/10.1002/dvdy.20491 |