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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites
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| Zeitschriftentitel: | Bioinformatics |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Bioinformatics, 31, 2015, 24, S. 4014-4016 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
Park, Jeongbin Bae, Sangsu Kim, Jin-Soo Park, Jeongbin Bae, Sangsu Kim, Jin-Soo |
|---|---|
| author |
Park, Jeongbin Bae, Sangsu Kim, Jin-Soo |
| spellingShingle |
Park, Jeongbin Bae, Sangsu Kim, Jin-Soo Bioinformatics Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites Computational Mathematics Computational Theory and Mathematics Computer Science Applications Molecular Biology Biochemistry Statistics and Probability |
| author_sort |
park, jeongbin |
| spelling |
Park, Jeongbin Bae, Sangsu Kim, Jin-Soo 1367-4811 1367-4803 Oxford University Press (OUP) Computational Mathematics Computational Theory and Mathematics Computer Science Applications Molecular Biology Biochemistry Statistics and Probability http://dx.doi.org/10.1093/bioinformatics/btv537 <jats:title>Abstract</jats:title> <jats:p>Summary: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.</jats:p> <jats:p>Availability and implementation: Free access at http://rgenome.net/cas-designer/.</jats:p> <jats:p>Contact: sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr</jats:p> <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p> Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites Bioinformatics |
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| title |
Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
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cas-designer: a web-based tool for choice of crispr-cas9 target sites |
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Computational Mathematics Computational Theory and Mathematics Computer Science Applications Molecular Biology Biochemistry Statistics and Probability |
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http://dx.doi.org/10.1093/bioinformatics/btv537 |
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2015 |
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4014-4016 |
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<jats:title>Abstract</jats:title>
<jats:p>Summary: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.</jats:p>
<jats:p>Availability and implementation: Free access at http://rgenome.net/cas-designer/.</jats:p>
<jats:p>Contact: sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr</jats:p>
<jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p> |
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| description | <jats:title>Abstract</jats:title> <jats:p>Summary: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.</jats:p> <jats:p>Availability and implementation: Free access at http://rgenome.net/cas-designer/.</jats:p> <jats:p>Contact: sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr</jats:p> <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p> |
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| spelling | Park, Jeongbin Bae, Sangsu Kim, Jin-Soo 1367-4811 1367-4803 Oxford University Press (OUP) Computational Mathematics Computational Theory and Mathematics Computer Science Applications Molecular Biology Biochemistry Statistics and Probability http://dx.doi.org/10.1093/bioinformatics/btv537 <jats:title>Abstract</jats:title> <jats:p>Summary: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.</jats:p> <jats:p>Availability and implementation: Free access at http://rgenome.net/cas-designer/.</jats:p> <jats:p>Contact: sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr</jats:p> <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p> Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites Bioinformatics |
| spellingShingle | Park, Jeongbin, Bae, Sangsu, Kim, Jin-Soo, Bioinformatics, Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites, Computational Mathematics, Computational Theory and Mathematics, Computer Science Applications, Molecular Biology, Biochemistry, Statistics and Probability |
| title | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
| title_full | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
| title_fullStr | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
| title_full_unstemmed | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
| title_short | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
| title_sort | cas-designer: a web-based tool for choice of crispr-cas9 target sites |
| title_unstemmed | Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites |
| topic | Computational Mathematics, Computational Theory and Mathematics, Computer Science Applications, Molecular Biology, Biochemistry, Statistics and Probability |
| url | http://dx.doi.org/10.1093/bioinformatics/btv537 |